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  <title>DIAGNOSA SURRA (TRYPANOSOMA EVANSI ) DENGAN METODE MODIFIKASI MHCT PADA DARAH KERBAU DI RPH BANDA ACEH DAN ACEH BESAR</title>
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  <place>
   <placeTerm type="text">Banda Aceh</placeTerm>
   <publisher>Universitas Syiah Kuala</publisher>
   <dateIssued>2019</dateIssued>
  </place>
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  <languageTerm type="code">id</languageTerm>
  <languageTerm type="text">Indonesia</languageTerm>
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 <note>DIAGNOSA SURRA (Trypanosom evansi) DENGAN METODE&#13;
MODIFIKASI MHCT PADA DARAH KERBAU DI RPH  &#13;
BANDA ACEH DAN ACEH BESAR &#13;
x&#13;
 &#13;
 &#13;
 &#13;
ABSTRAK&#13;
 &#13;
Penelitian ini bertujuan untuk mendeteksi Trypanosoma evansi pada darah&#13;
kerbau yang di potong di RPH Banda Aceh dan Aceh Besar menggunakan metode&#13;
modifikasi MHCT (microhematocrit centrifugation technique) dengan mengambil&#13;
buffy coat pada darah yang disentrifus. Sebanyak 50 sampel darah segar kerbau&#13;
yang dicampur dengan antikoagulan masing-masing disentrifus sebanyak 1 ml&#13;
dengan kecepatan 3000 g. Buffy coat  yang terbentuk dimasukkan ke dalam&#13;
tabung eppendorf&#13;
®&#13;
 lalu dihomogenkan dan dimasukkan ke dalam tabung&#13;
hematokrit sebanyak&#13;
 3&#13;
?&#13;
tabung kemudian disentrifus dengan kecepatan 12.000&#13;
rpm. Hasil penelitian menunjukkan bahwa metode secara parasitologi yaitu&#13;
metode modifikasi MHCT memiliki kemampuan mendiagnosa lebih sensitif dan&#13;
mendapatkan jumlah T. evansi yang lebih banyak daripada metode MHCT dan&#13;
metode ulas darah tipis. Berdasarkan penelitian tersebut dapat disimpulkan bahwa&#13;
metode modifikasi MHCT lebih sensitif untuk mendiagnosa T. evansi daripada&#13;
metode MHCT dan metode ulas darah tipis. &#13;
4&#13;
 &#13;
&#13;
Kata kunci: kerbau, Trypanosoma evansi, MMHCT, MHCT, UDT &#13;
  &#13;
SURRA DIAGNOSIS (Trypanosoma evansi) WITH  MODIFIED MHCT&#13;
METHOD IN BUFFALOES BLOOD AT BANDA ACEH AND  &#13;
ACEH BESAR SLAUGHTERHOUSE&#13;
 &#13;
xi&#13;
 &#13;
ABSTRACT&#13;
 &#13;
This study was aim to detected Trypanosoma evansi in buffaloes blood&#13;
that slaughtered in Banda Aceh and Aceh Besar slaughteredhouse using MHCT&#13;
modified method (Microhematocrit Centrifugation Technique) by taking buffy&#13;
coat of centrifuged blood. Fifty samples of whole blood mixed with&#13;
anticoagulants (EDTA) and centrifuged 1 mL respectively at 3000 g speed. Buffy&#13;
coat moved into the eppendorf tube then homogenized, and inserted into&#13;
hematocrite tube until ¾ part and then centrifuged with 12.000 rpm. The results&#13;
showed that the parasitological method especially the modifiied method of&#13;
MHCT, had ability to diagnose better and obtain a greater number of T. evansi&#13;
compared with the MHCT method and giems stained blood smears method. Based&#13;
on the studies it can be concluded that the modified MHCT method was more&#13;
sensitive for diagnosing T. evansi than the MHCT method and giemsa smear&#13;
method.&#13;
&#13;
Keyword: buffaloes, MMHCT, MHCT, Giemsa stained blood smears &#13;
&#13;
&#13;
 &#13;
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  <physicalLocation>ELECTRONIC THESES AND DISSERTATION Universitas Syiah Kuala</physicalLocation>
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