Electronic Theses and Dissertation

ABSTRAK
Enzim selulase dapat diisolasi dari bakteri termofil yang diperoleh dari sumber mata air panas Ie Seuum, Aceh Besar. Penelitian ini bertujuan untuk mendapatkan isolat, karakterisasi morfologi, menentukan isolat bakteri termofil yang mampu menghasilkan enzim selulase, dan mengetahui karakter biokimia isolat terpilih. Metode pengambilan sampel pada penelitian ini dilakukan dengan purposive sampling pada suhu 70 oC, 60 oC dan 50 oC. Isolasi bakteri termofil dilakukan pada media Nutrient Agar (NA). Hasil isolasi bakteri termofil yang diperoleh adalah empat isolat pada suhu 70 oC, lima isolat pada suhu 60 oC, dan tujuh isolat pada suhu 50 oC. Dari 18 isolat yang didapatkan, 15 diantaranya mampu menghasilkan enzim selulase. Produksi enzim selulase dapat diketahui dengan adanya zona bening di sekitar koloni bakteri pada media CMC setelah ditetesi congo red 1% dan dicuci NaCl 1M. Nilai Indeks Selulolitik (IS) lima tertinggi diperoleh dari isolat BIS75; BIS64; BIS52; BIS54; BIS56 yaitu 1,23; 2,22; 1,39; 1,59; 1,10. Pengujian biokimia dilakukan terhadap 5 isolat yang memiliki nilai indeks selulolitik tertinggi. Hasil karakterisasi menunjukkan bahwa isolat bakteri tersebut diduga termasuk dalam genus Bacillus sp..

Kata Kunci : Bakteri termofil, enzim selulase, sumber air panas, Ie Seuum

ABSTRACT
Cellulase enzymes can be isolated from thermophile bacteria obtained from the hot spring Ie Seuum, Aceh Besar. This research aimed to obtain isolates, morphological characterization, determine thermophile bacterial isolates capable of producing cellulase enzymes, and determine the biochemical characteristict of selected isolates. The sampling method in this research was conducted by a purposive sampling at temperature of 70 oC, 60 oC and 50 oC. Isolation of thermophilic bacteria was carried out on Nutrient Agar (NA) media. The results of the isolation of thermophilic bacteria were four isolates isolated at 70 oC, five isolates at 60 oC, and seven isolates at 50 oC. Of the 18 isolates obtained, 15 of them were able to produce cellulase enzymes. Cellulase enzyme production can be determined by the presence of clear zones around bacterial colonies on CMC media after 1% congo red drops and wash with 1 M NaCl. The highest five Cellulolytic Index (IS) values were obtained from isolates BIS75; BIS64; BIS52; BIS54; BIS56 that were 1.23; 2.22; 1.39; 1.59; 1.10, respectively. Biochemical tests were carried out on 5 isolates which had the highest cellulolytic index values. The characterization results showed that the bacterial isolate were suspected to be included in the genera Bacillus sp..

Keyword: Thermophilic bacteria, cellulase enzymes, hot springs, Ie Seuum

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