Electronic Theses and Dissertation

ABSTRAK
Uji aktivitas antibakteri ekstrak etanol daging dan kulit buah pisang klutuk muda
(Musa balbisiana Colla BB) terhadap bakteri Staphylococcus aureus dan Escherichia
coli telah dilakukan untuk mengetahui aktivitas ekstrak etanol daging dan kulit buah
pisang klutuk muda dalam menghambat pertumbuhan bakteri tersebut. Ekstrak dari
sampel diperoleh melalui metode maserasi dengan pelarut etanol 96%. Simplisia
daging dan kulit buah masing-masing mengandung kadar air sebesar 5% dan 7,17%,
kadar abu total 14,55% dan 11,61%, kadar sari larut air 10,67% dan 9,67%, dan kadar
sari larut etanol 9,67% dan 9,67%. Ekstrak etanol daging buah dan kulit buah masingmasing
mengandung kadar air sebesar 28,26% dan 15,5%, kadar abu total 10,16% dan
14,09%, kadar sari larut air 33,33% dan 71,67%, dan kadar sari larut etanol 69,16%
dan 78,33%. Hasil uji fitokimia menunjukkan bahwa kedua ekstrak mengandung
senyawa alkaloid, flavonoid, tanin, triterpenoid dan saponin. Uji aktivitas antibakteri
ekstrak etanol sampel dilakukan dengan menggunakan metode sumuran dengan variasi
konsentrasi ekstrak yaitu 5; 10; 20; 30 dan 40%. Aktivitas antibakteri terhadap S.
aureus pada ekstrak etanol kulit buah lebih tinggi dibandingkan dengan aktivitas
ekstrak etanol daging buah. Sedangkan aktivitas antibakteri terhadap bakteri E. coli
menunjukkan ekstrak etanol daging buah memiliki aktivitas lebih baik. Aktivitas
tertinggi ekstrak etanol kulit buah terhadap S. aureus diperoleh pada konsentrasi 10%
dengan zona hambat 9,3 mm dan aktivitas tertinggi ekstrak etanol daging buah
terhadap E. coli diperoleh pada konsentrasi 20% dengan zona hambat 8,63 mm.
Kata kunci: Buah pisang klutuk muda, Musa balbisiana Colla BB, Ekstrak etanol,
antibakteri.

ABSTRACT
Antibacterial activity test of ethanol extract from flesh and peel of unripe klutuk
banana fruit (Musa balbisiana Colla BB) towards bacterial of Staphylococcus aureus
and Escherichia coli has been conducted to know the activity of ethanol extract from
flesh and peel of unripe klutuk banana fruit in inhibiting the growth of those bacterials.
The extract of the samples were obtained through maceration method by ethanol 96%
as a solvent. Flesh and peel of fruit simplicial characterization contains water content
of 5% and 7,17%, total ash content of 14,55% and 11,61%, water soluble extract
content of 10,67% and 9,67%, and ethanol soluble extract content of 9,67% and
9,67%. Ethanol extract from flesh and peel of unripe klutuk banana fruit contains
water content of 28,26% and 15,5%, total ash content of 10,16% and 14,09%, water
soluble extract content of 33,33% and 71,67%, and ethanol soluble extract content of
69,16% and 78,33%. Phytochemical test showed that both of extract contained
alkaloid compounds, flavonoids, tannins, triterpenoids and saponins. Antibacterial
activity test of ethanol extract from samples was conducted using a well diffusion
method with variation of extract concentrations of 5; 10; 20; 30 and 40%.
Antibacterial activity towards bacterial of S. aureus at ethanol extract from peel of
fruit activity is higher than activity of ethanol extract from flesh of fruit. While
antibacterial activity towards bacterial of E. coli from flesh of fruit showed better
activity. The highest activity of ethanol extract from peel of fruit towards S. aureus
were obtained at 10% concentration with inhibiting zone diameter of 9,3 mm and the
highest activity from ethanol extract from flesh of fruit towards E. coli were obtained
at 20% concentration with inhibiting zone diameter of 8,63 mm.
Keywords: Unripe Klutuk banana fruit, Musa balbisiana Colla BB, ethanol extract,
antibacterial.

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