Electronic Theses and Dissertation

IDENTIFIKASI KUALITAS EMBRIO SAPI DONOR
DI BALAI EMBRIO TERNAK (BET) CIPELANG
BOGOR, JAWA BARAT
ABSTRAK
Transfer embrio merupakan salah satu teknologi reproduksi untuk meningkatkan mutu genetik ternak dalam waktu yang singkat. Keberhasilan aplikasi TE di lapangan perlu didukung oleh berbagai faktor, salah satunya adalah kualitas embrio yang digunakan. Kegiatan produksi embrio secara in vivo dilakukan dengan beberapa tahapan yaitu, seleksi donor, superovulasi, singkronisasi estrus, inseminasi buatan, flushing embrio dan evaluasi embrio. Kegiatan evaluasi embrio meliputi, filter embrio, pencarian embrio, koleksi embrio, grading embrio, dan penilaian kualitas embrio. Kegiatan ini bertujuan untuk mengetahui kualitas embrio sapi donor yang ada di Balai Embrio Ternak Cipelang, Bogor. Persentase jumlah embrio yang dikoleksi dari bangsa sapi donor yaitu 2 ekor FH, 2 ekor simmental, 4 ekor limousin, 2 ekor angus, dan 2 ekor Belgian blue secara berturut adalah 88.89%; 91.67%; 89.04%; 28.57% dan 66.67%. Persentase embrio layak transfer dari jumlah embrio yang dikoleksi secara berurut adalah 62.50%, 90.91%, 80.00%, 0.00% dan 50.00%. Produksi embrio secara In vivo di Balai Embrio Ternak (BET) Cipelang dilakukan dengan superovulasi FSH dan PMSG. Hasil evaluasi kualitas embrio hasil flushing dari berbagai induk donor di BET Cipelang 92 embrio dengan grade 1, 39 (42.39%); grade 2, 31(33.69%); grade 3, 9 (9.78%) dan grade 4, 13 (4.34%). Kesimpulan: Embrio yang layak transfer berjumlah 70 embrio (76.08%) dan yang tidak layak transfer sebanyak 18 embrio (19.56%).

Kata kunci: sapi donor, produksi embrio, flushing embrio, kualitas embrio






IDENTIFICATION OF EMBRYO QUALITY OF DONOR COW
IN BALAI EMBRIO TERNAK (BET) CIPELANG
BOGOR, JAWA BARAT
ABSTRACT

Embrio Transfer (ET) is one of the reproductive technology to enhance genetic merit of livestock in short period of time. The efficiency of ET application in the field is affected by several factors, one of the factors is the embryo quality produced. The in vivo production was performed in several levels; donor selection, superovulation, oestrus synchronization, artificial insemination, embryo flushing, and evaluation. In evaluation of embryo, the procedure includes embryo filtration, searching, collecting, grading and evaluation. This activity aims to determine the quality of donor cow embryos at Balai Embrio Ternak Cipelang. Data collected from the evaluation of post flushing embryos of the donor namely two headsof FH, two headsof Simmental, four headsof Limousine, two headsof Angus and two headsof Belgian Blue breed, was respectively 88.89%; 91.67%; 89.04%; 28.45%; and 66.67%. The percentage of qualified embryo to be transferred from the total collection was respectively 62.50%; 91.90%; 80.00%; 0.00%; and 50.00%. Production of embryo by in vivo fertilization in BET Cipelang was performed using FSH and PMSG to induce superovulation. The result of 92 embryos graded was grade 1: 39 embryo (42.39%); grade 2: 31 embryo (33.69%); grade 3: 9 embryo (9.97%); and grade 4: 13 embryo (4.34%). Conclusion 70 embryos qualified to be transferred (76.08%) and 18 embryos (19.56%) were under qualified.

Keyword : Donor cattle, embryo production, embryo flushing, embryo quality.

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