SRI RAHAYU Primary Author mixed material bibliography Banda Aceh Program Studi Magister Biologi Universitas Syiah Kuala 2019 id Indonesia

Theses

ABSTRAK Aktinobakteri merupakan bakteri yang mampu menghasilkan metabolit sekunder yang sama dengan tanaman inangnya dan berpotensi dijadikan sebagai obat. Penelitian ini bertujuan untuk melihat potensi aktinobakteri endofit asal jahe sebagai inhibitor lipase pankreas serta nilai toksisitasnya. Aktinobakteri endofit dari jahe diisolasi menggunakan media Humic acid Vitamin (HV). Aktivitas inhibitor lipase pankreas diuji menggunakan lipase L321 Sigma diukur dengan menggunakan Spektrofotometer. Nilai toksisitas diuji menggunakan metode Brine Shrimp Lethality Test (BSLT) dengan menghitung nilai LC50 dan LT50 menggunakan analisis probit. Aktivitas inhibitor tertinggi terdapat pada isolat AJ2 dan AJ4 dengan nilai inhibisi sebesar 77,4% dan 89,8%. Nilai LC50 isolat AJ2 dan AJ4 adalah 309,358 µg/ml dan 653,381 µg/ml. Nilai LT50 kedua isolat adalah adalah 11,879 jam dan 17,569 jam. Hasil fitokimia ekstrak etanol AJ2 dan AJ4 terdeteksi mengandung terpenoid, fenol, tanin, flavonoid, alkaloid dan saponin. Kata Kunci: aktinobakteri endofit, jahe, inhibitor lipase pankreas, toksisitas. ABSTRACT Actinobacteria is a bacterium that capable of produce secondary metabolites that are the same as their host plants and potentially used as medicine. This study aims to see the potency of endophytic actinobacteria from ginger as pancreatic lipase inhibitor and their toxicity value. Endophytic actinobacteria from ginger were isolated using Humic acid Vitamin (HV) media. The pancreatic lipase inhibitor activity tested using L321 Sigma lipase was measured using a spectrophotometer. Toxicity values were tested using the Brine Shrimp Lethality Test (BSLT) method by calculating the LC50 and LT50 values using probit analysis. The highest inhibitor activity was found in AJ2 and AJ4 isolates with inhibition values of 77,4% and 89,8%. The LC50 values of AJ2 and AJ4 isolates were 309,358 µg / ml and 653,381 µg / ml. The LT50 values of the two isolates were 11,879 hours and 17,569 hours. The phytochemical results of AJ2 and AJ4 ethanol extracts were detected containing terpenoid, phenol, tannin, flavonoid, alkaloid and saponin. Keywords: endophytic actinobacteria, ginger, pancreatic lipase inhibitor, toxicity MEDICINAL PLANTS GINGER - BOTANY 584.39 ELECTRONIC THESES AND DISSERTATION Universitas Syiah Kuala 59444 2019-06-24 16:11:41 2019-10-04 15:56:49 machine generated