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  <title>EFEK PERBEDAAN WAKTU PENGAMBILAN SAMPEL DARAH DAN FESES TERHADAP KOSENTRASI HORMON KORTISOL PADA SAPI ACEH</title>
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 <name type="Personal Name" authority="">
  <namePart>AHMAD WAHYUDI</namePart>
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   <roleTerm type="text">Primary Author</roleTerm>
  </role>
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  <place>
   <placeTerm type="text">Banda Aceh</placeTerm>
   <publisher>Universitas Syiah Kuala</publisher>
   <dateIssued>2018</dateIssued>
  </place>
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  <languageTerm type="code">id</languageTerm>
  <languageTerm type="text">Indonesia</languageTerm>
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 <note>EFEK PERBEDAAN WAKTU PENGAMBILAN SAMPEL DARAH DAN&#13;
FESES TERHADAP KONSENTRASI HORMON KORTISOL &#13;
 PADA SAPI ACEH &#13;
ABSTRAK&#13;
Salah satu parameter yang harus dievaluasi sebelum dilakukan analisis hormon &#13;
kortisol adalah waktu koleksi sampel. Hal ini karena, terdapat variasi diurnal&#13;
konsentrasi kortisol pada pagi dan siang hari. Oleh karena itu, penelitian ini bertujuan&#13;
untuk mengukur konsentrasi kortisol di dalam darah dan feses yang dikoleksi pada pagi&#13;
dan siang hari untuk mengevaluasi ada tidaknya variasi diurnal pada sapi aceh. Sampel&#13;
yang digunakan dalam penelitian ini adalah 16 sampel darah dan feses (8 sampel di&#13;
pagi hari dan 8 sampel di siang hari) yang dikoleksi dari 8 sapi aceh betina umur 2-3&#13;
tahun. Sampel darah dipreparasi menjadi plasma, sedangkan sampel feses diekstrak&#13;
selanjutnya dilakukan pengukuran hormon kortisol menggunakan teknik enzymelinked&#13;
immunosorbent&#13;
assay&#13;
&#13;
(ELISA). Data yang diperoleh diuji menggunakan uji-t.&#13;
Hasil penelitian menunjukkan  konsentrasi hormon kortisol di dalam plasma pada pagi&#13;
hari secara signifikan lebih tinggi 79,34% dibandingkan siang hari (P0,05). Kesimpulan, terdapat variasi diurnal hormon kortisol pada plasma&#13;
sapi aceh pada pagi dan siang hari, tetapi pada sampel feses tidak terlihat variasi&#13;
diurnal.&#13;
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xi &#13;
 &#13;
The Effect of Different Time of Sample Collection of Blood and Feces on the&#13;
Cortisol Concentration in Aceh Cattle &#13;
&#13;
ABSTRACT &#13;
One of the parameter that must be evaluated prior to perform the cortisol&#13;
hormone analysis is time of sample collection. This is due to there is diurnal variation&#13;
of cortisol concentrations in the morning and noon. Therefore,this study aims to&#13;
measure cortisol concentration in the blood and feces collected in the morning and&#13;
noon for evaluating the diurnal variation in aceh cattle. Samples used in this study&#13;
were 16 blood and feces samples (8 samples in the morning and 8 samples in the noon)&#13;
collected from eight adult females of aceh cattle age 2-3 years old.  Blood samples&#13;
were prepared into a plasma, while the feces samples were extracted and then&#13;
performed cortisol hormone measurement through enzyme-linked immunosorbent&#13;
assay (ELISA) technique. Data were analyzed by using t-test. The result showed that &#13;
concentrations of cortisol  in the plasma collected in the morning were significantly&#13;
higher  79,34%  compared to the noon samples (p  0,05).&#13;
In conclusion, there is a variation diurnal in plasma cortisol of aceh cattle, but there&#13;
is no variation diurnal in feces cortisol of aceh cattle. &#13;
 &#13;
xii</note>
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