AYANATUN HUSNA Primary Author mixed material bibliography Banda Aceh FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM UNIVERSITAS SYIAH KUALA 2017 id Indonesia

Skripsi

ABSTRAK Resistensi antibiotik terhadap bakteri mendorong dilakukannya berbagai penelitian untuk mendapatkan bahan baku obat yang berasal dari alam sebagai antibakteri. Penelitian ini menguji aktivitas antibakteri ekstrak n-heksana, etil asetat, dan metanol daun peria laut (Colubrina asiatica (L.) Brongn) terhadap bakteri Methicillin Resistants Staphylococcus aureus (MRSA). Ekstraksi daun peria laut dilakukan dengan metode maserasi bertingkat menggunakan ekstrak n-heksana, etil asetat, dan metanol. Terhadap masing-masing ekstrak dilakukan uji fitokimia dan karakterisasi ekstrak. Uji aktivitas antibakteri dilakukan menggunakan metode Kirby-Bauer dengan variasi konsentrasi 5, 10, 15, dan 20%. Hasil uji fitokimia menunjukkan bahwa ekstrak n-heksana tidak memberikan hasil positif terhadap golongan senyawa alkaloid, saponin, tanin, flavonoid, dan steroid/triterpenoid. Ekstrak etil asetat mengandung senyawa saponin dan steroid. Ekstrak metanol mengandung senyawa tanin, flavonoid, saponin dan steroid. Hasil karakteristik ekstrak n-heksana, etil asetat dan metanol menunjukkan kadar air sebesar 6,70; 32,11; dan 16,52%, kadar sari larut air sebesar 4,31; 2,65; dan 16,52%, kadar sari larut etanol sebesar 8,28; 56; dan 54,52%, kadar abu total sebesar 0,14; 6,37; dan 4,83%. Hasil uji aktivitas antibakteri menunjukkan bahwa ekstrak n-heksana dan metanol tidak memiliki zona hambat, sedangkan ekstrak etil asetat menunjukkan zona hambat terbesar pada konsentrasi 20% dengan ratarata diameter zona hambat 10,49 mm. Kata kunci: Colubrina asiatica (L.) Brongn, Methicillin Resistants Staphylococcus aureus, dan metode Kirby-Bauer. ABSTRACT Antibiotic resistence on Bacterial encourages varicus research to find raw material drugs from nature as antibacterial. Antibacterial activity test of n-hexane, ethyl acetate, and methanol extract of peria laut leaves was conducted againts Metichillin Resistant Staphylococcus aureus (MRSA). Extraction was accomplished using solvent multistep maceration method by n-hexana, ethyl acetate, and methanol. The charactetization of extract and phytochemical test were evaluated for peria laut leaves extract. Antibacterial activity was determined by Kirby-Bauer method with variation of concentration 5, 10, 15, and 20%. Phytochemical test results showed that n-hexane extract was not give positive result on alkaloid, saponin, tannin, flavonoid and steroid/triterpenoid. Ethyl acetate extract contains saponin and steroid compound. Methanol extract contains tannin, flavonoid, and steroid. Characterization extract n-hexane, ethyl acetate, and methanol extract showed that water content 6.70; 32.11; and 16.52%, water soluble extract were 4.31; 2.65; and 54.52%, ethanol soluble extract 8.28; 56.26; and 54.53%, and total ash value 0.14; 6.37; and 4.83%. The results of antibacterial activity test showed that n-hexane and methanol extract were not present inhibition zone, however ethyl acetate extract showed that the largest inhibition zone on 20% concentration with average diameter was 10,49 mm. Keywords: Colubrina asiatica (L.) Brongn, Metichillin Resistant Staphylococcus aureus, and Kirby-Bauer method. MEDICINAL PLANTS ANTIBIOTICS-FHARMACOLOGY 1 ELECTRONIC THESES AND DISSERTATION Universitas Syiah Kuala 35639 2017-10-25 11:44:14 2018-10-30 08:47:19 machine generated