Electronic Theses and Dissertation

ABSTRAK
Media buatan kultur in vitro merupakan media alternatif pengganti media
Murashige dan Skoog (MS) yang umum digunakan seperti Growmore.
Penambahan air kelapa dalam media dapat mempengaruhi pertumbuhan tanaman,
sehingga perlu dilakukan penelitian tentang pengaruh penambahan beberapa
konsentrasi air kelapa dalam media buatan terhadap pertumbuhan krisan
(Dendranthema grandiflora). Penelitian ini dilaksanakan di Laboratorium Biologi
Sel dan Molekuler Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan
Alam, Universitas Syiah Kuala, Banda Aceh dari bulan April sampai Juli 2013.
Penelitian ini menggunakan Rancangan Acak Kelompok Sub Sampling dengan
media sebagai kelompok dan air kelapa sebagai perlakuan. Pengamatan dilakukan
mulai dari penanaman eksplan sampai satu bulan. Hasil penelitian menunjukkan
tidak ada perbedaan nyata antara penggunaan media MS dan media buatan dengan
beberapa konsentrasi air kelapa terhadap inisiasi tunas, akar, serta pertumbuhan
daun. Perlakuan K1 (2,5% air kelapa dalam media) menunjukkan perbedaan nyata
dengan K0 (0% air kelapa dalam media) terhadap tinggi batang krisan.
Pertumbuhan panjang akar krisan terbaik ditunjukkan pada media A (MS) yaitu
4,8125 cm, sama halnya dengan pertumbuhan planlet (10,5042 cm), akan tetapi
pertumbuhan planlet tidak berbeda nyata dengan pertumbuhan pada media D
(Growmore 1 gram/L) yaitu 9,1750 cm.
Kata kunci: Dendranthema grandiflora, air kelapa, media MS, media buatan
ABSTRACT
Artificial medium for in vitro culture is an alternative media to subtitute
Murashige and Skoog (MS). Growmore added with organic substances as coconut
water can be used as artificial medium. Coconut water can affect plant growth, so
the research is to investigate the influence of coconut water in different
concentration to chrysanth (Dendranthema grandiflora)’s growth should be done.
The research was conducted in Laboratory of Molecular and Cell Biology,
Department of Biology, Faculty of Mathematics and Natural Sciences, Syiah
Kuala University Darussalam, Banda Aceh, from April until July 2013. The
research was designed by using Randomized Block Design with sub sampling.
Media act as group, while coconut water concentration as treatment. Observation
was started from growing time up to a month after planting. The result showed
that there was no significant different between using MS and artificial medium
with different coconut water concentration to initiate shootlet and rootlet, as well
as the leaf growth. For chrysant hight, treatment K1 (2.5% of coconut water in
medium) was different significantly from K0 (0% of coconut water in medium).
The best growth of chrysant’s root presented in medium A, that was 4.8125 cm.
Furthermore, the best growth of planted was in medium A as well, but it was not
significantly different from one in Medium D (Growmore 1 gram/L), that is
9.1750 cm.
Key words: Dendranthema grandiflora, coconut water, MS medium, artificial
medium

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