Electronic Theses and Dissertation

ABSTRAK

Nama : Vivi ZayantiProgram Studi : Kedokteran GigiJudul : Kemampuan Porphyromonas gingivalis Mempengaruhi
Pertumbuhan Candida albicans dalam Berbagai pH Saliva Buatan

Kandidiasis rongga mulut dan periodontitis kronis termasuk penyakit infeksi rongga mulut yang masing-masing disebabkan oleh Candida albicans (C. albicans) dan Porphyromonas gingivalis (P. gingivalis). Dua penyakit oral ini dapat dicegah dengan berbagai pendekatan salah satunya dengan mempelajari siklus ekologi kedua mikroorganisme tersebut. Tujuan penelitian ini yaitu menganalisis pengaruh P. gingivalis tehadap pertumbuhan C. albicans dengan pendekatan pH saliva. Suspensi dari kedua mikroorganisme diinteraksikan kedalam saliva dengan pH 4, 5, 6, 7, 8, dan 9 selama 72 jam. Metode kultur slide juga dilakukan untuk mengamati struktur morfologi C. albicans. Perubahan pH saliva dan jumlah koloni dihitung menggunakan pH meter dan colony counter. Data hasil perubahan pH dan data jumlah koloni dianalisis dengan statistik ANOVA dengan p = 0,05. Berdasarkan hasil penelitian dapat disimpulkan bahwa P. gingivalis memiliki pengaruh terhadap pertumbuhan C. albicans yang ditandai dengan perubahan pH saliva menjadi basa dan jumlah koloni P. gingivalis yang lebih dominan dari jumlah koloni C. albicans.

Kata kunci: kandidiasis rongga mulut, periodontitis kronis, C. albicans, P. gingivalis, pH saliva.

ABSTRACT

Name : Vivi ZayantiPrograme of study : DentistryTitle : The Ability of Porphyromonasgingivalis in Influencingthe
Growth of Candida albicans in Artificial Saliva with Any pHs

Oral candidiasis and chronic periodontitis are oral infection diseases which are caused by Candida albicans (C. albicans) and Porphyromonasgingivalis (P. gingivalis)respectively. These two oral diseases can be prevented by using various approaches. One of them is by learning ecology cycle of the two microorganisms. The objective of this research was to analyze the influence of P. gingivalis on the growth of C. albicans with pH of saliva approach. The suspensions of the two microorganisms are given contact two pH of 4, 5, 6, 7, 8, and 9 in 72 hours. Culture slide method was also used to reveal the morphological structure of C. albicans. The changes in the numbers of pH of saliva and colony were measured by using a pH meter and colony counter. The data obtained from the changes in the numbers of pH of saliva and colony were analyzed by using ANOVA with p = 0.05. The statistical analysis showed that there was no significant difference between pHs at the same time and at different time. The results of statistical analysis for number of colony there was significant difference both between pHs and between the two organisms. Based on the research results, it can be concluded that P. gingivalis influences the growth of C. albicans which is identified by the change in pH of saliva which becomes base and number of P. gingivalis colony is more dominant than that of C. albicans.

Keywords: oral candidiasis, chronic periodontitis, C. albicans, P. gingivalis, pH of saliva

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