Electronic Theses and Dissertation

Penelitian ini bertujuan untuk mensintesis nanopartikel tembaga (Cu) menggunakan ekstrak metanol daun glodokan tiang (Polyalthia longifolia) melalui metode green synthesis yang dikombinasikan dengan sonikasi, serta mengevaluasi karakteristik dan aktivitas biologinya. Hasil analisis menunjukkan bahwa ekstrak metanol daun glodokan tiang mengandung Total Phenolic Content (TPC) sebesar 69,17 mg GAE/g ekstrak dan Total Flavonoid Content (TFC) sebesar 20,5 mg QE/g ekstrak. Keberhasilan sintesis nanopartikel Cu-PLLM ditunjukkan oleh perubahan warna larutan dan dikonfirmasi melalui karakterisasi menggunakan UV-Vis, FTIR, XRD, SEM-EDS, dan TEM. Analisis XRD mengidentifikasi terbentuknya fase kristal Cu, Cu₂O, dan CuO, sedangkan hasil TEM menunjukkan morfologi partikel yang cenderung kuasi-sferis hingga tidak beraturan dengan ukuran dominan pada kisaran 3–4 nm. Pengujian aktivitas antijamur terhadap Candida albicans menggunakan metode difusi cakram menunjukkan bahwa nanopartikel Cu-PLLM mampu menghambat pertumbuhan jamur pada seluruh konsentrasi yang diuji, dengan daya hambat tertinggi diperoleh pada konsentrasi 5%. Secara keseluruhan, hasil penelitian menunjukkan bahwa ekstrak metanol daun glodokan tiang berpotensi dimanfaatkan sebagai agen pereduksi dan penstabil dalam green synthesis nanopartikel tembaga
yang memiliki aktivitas antijamur
Kata kunci: green synthesis, nanopartikel tembaga, Polyalthia longifolia, sonikasi, antijamur

This study aimed to synthesize copper nanoparticles (CuNPs) using methanolic extract of glodokan tiang (Polyalthia longifolia) leaves through a green synthesis method combined with sonication, and to evaluate their characteristics and biological activity. The analysis showed that the methanolic leaf extract contained a Total Phenolic Content (TPC) of 69.17 mg GAE/g extract and a Total Flavonoid Content (TFC) of 20.5 mg QE/g extract. The successful synthesis of Cu-PLLM nanoparticles was indicated by a color change in the reaction solution and confirmed through characterization using UV–Visible spectroscopy (UV–Vis), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscopy coupled with Energy Dispersive X-ray Spectroscopy (SEM–EDS), and Transmission Electron Microscopy (TEM). XRD analysis confirmed the formation of Cu, Cu₂O, and CuO crystalline phases, while TEM analysis revealed quasi-spherical to irregular-shaped nanoparticles with a predominant particle size of 3–4 nm. Antifungal activity against Candida albicans was evaluated using the disk diffusion method, and the Cu-PLLM nanoparticles exhibited inhibitory activity at all tested concentrations, with the highest inhibition zone observed at the 5% concentration. Overall, the results demonstrate that the methanolic extract of P. longifolia leaves has potential as both a reducing and capping agent for the green synthesis of copper nanoparticles with promising antifungal activity. Keywords: green synthesis, copper nanoparticles, Polyalthia longifolia, sonication, antifungal