Electronic Theses and Dissertation

Paparan sinar ultraviolet B (UVB) merupakan salah satu faktor ekstrinsik utama yang menyebabkan penuaan dini pada kulit melalui peningkatan pembentukan reactive oxygen species (ROS), sehingga mengakibatkan penurunan kelembapan, elastisitas, dan kadar kolagen kulit. Daun cempaka kuning (Magnolia champaca L.) diketahui mengandung senyawa antioksidan yang berpotensi menghambat proses penuaan kulit. Penelitian ini bertujuan untuk mengetahui efektivitas krim ekstrak etil asetat daun cempaka kuning sebagai agen anti-aging pada kulit mencit yang diinduksi sinar UVB serta menentukan konsentrasi ekstrak yang paling efektif. Penelitian menggunakan rancangan acak lengkap (RAL) dengan tujuh perlakuan, yaitu kontrol normal (K0), kontrol negatif (K-), kontrol positif (K+), basis krim (P1), serta krim ekstrak dengan konsentrasi 0,267% (P2), 0,534% (P3), dan 1,068% (P4), masing-masing terdiri atas tiga ulangan. Parameter yang diamati meliputi kelembapan, elastisitas, dan kadar kolagen kulit menggunakan skin analyzer EH-900U. Data dianalisis menggunakan One-Way ANOVA, Welch ANOVA, uji Tukey HSD, dan Games-Howell pada taraf signifikansi 5%. Hasil penelitian menunjukkan bahwa pemberian krim ekstrak daun cempaka kuning berpengaruh terhadap kadar kelembapan kulit, tetapi tidak berpengaruh signifikan terhadap elastisitas dan kadar kolagen kulit. Konsentrasi ekstrak 0,267% (P2) merupakan konsentrasi yang paling efektif dalam meningkatkan kelembapan kulit. Berdasarkan hasil penelitian dapat disimpulkan bahwa krim ekstrak etil asetat daun cempaka kuning berpotensi sebagai agen anti-aging, terutama dalam meningkatkan kadar kelembapan kulit, sedangkan konsentrasi ekstrak yang digunakan belum memberikan peningkatan yang signifikan terhadap elastisitas dan kadar kolagen kulit.


Kata Kunci: Magnolia champaca L., anti-aging, sinar UVB, kelembapan, elastisitas, kolagen.

Ultraviolet B (UVB) exposure is one of the major extrinsic factors contributing to premature skin aging by increasing the production of reactive oxygen species (ROS), resulting in decreased skin moisture, elasticity, and collagen levels. Yellow champaca (Magnolia champaca L.) leaves contain antioxidant compounds with potential anti-aging properties. This study aimed to evaluate the effectiveness of an ethyl acetate extract cream of yellow champaca leaves as an anti-aging agent on UVB-induced mouse skin and to determine the most effective extract concentration. A completely randomized design (CRD) was employed using seven treatment groups: normal control (K0), negative control (K−), positive control (K+), cream base (P1), and extract cream concentrations of 0.267% (P2), 0.534% (P3), and 1.068% (P4), with three replicates per group. Skin moisture, elasticity, and collagen levels were evaluated using an EH-900U skin analyzer. Data were analyzed using One-Way ANOVA, Welch ANOVA, Tukey HSD, and Games-Howell tests at a 5% significance level. The results showed that the ethyl acetate extract cream of yellow champaca leaves significantly affected skin moisture but did not significantly affect skin elasticity or collagen levels. The 0.267% extract concentration (P2) was the most effective in improving skin moisture. It can be concluded that the ethyl acetate extract cream of yellow champaca (Magnolia champaca L.) leaves has potential as an anti-aging agent, particularly in improving skin moisture, although the extract concentrations used did not produce significant improvements in skin elasticity or collagen levels. Keywords: Magnolia champaca L., anti-aging, UVB irradiation, skin moisture, elasticity, collagen.