Electronic Theses and Dissertation

Kulit wajah merupakan bagian tubuh yang paling sering terpapar radikal bebas yang dapat menyebabkan kerusakan kulit seperti kusam, perubahan warna, dan penuaan dini. Antioksidan berperan dalam menangkal radikal bebas dengan mendonorkan elektron sehingga menghambat reaksi oksidatif berantai. Salah satu tanaman yang berpotensi sebagai sumber antioksidan adalah daun sirih cina (Peperomia pellucida L. Kunth). Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak etanol 80% daun sirih cina menggunakan metode DPPH serta mengevaluasi karakteristik fisik sediaan facial wash gel yang diformulasikan dari ekstrak tersebut. Simplisia dibuat melalui pengeringan suhu ruang selama 30 hari dan diekstraksi menggunakan metode maserasi dengan etanol 80% (1:10), menghasilkan rendemen ekstrak 13,39%. Skrining fitokimia menunjukkan adanya alkaloid, flavonoid, saponin, tanin, triterpenoid, dan steroid. Uji antioksidan dengan metode DPPH menggunakan spektrofotometer UV-Vis pada 515 nm menunjukkan nilai IC₅₀ sebesar 49,81 ppm (kategori sangat kuat), sedangkan vitamin C sebagai kontrol positif memiliki IC₅₀ sebesar 1,2 ppm. Ekstrak diformulasikan menjadi facial wash gel dengan variasi konsentrasi F0 (0%), F1 (0,5%), F2 (1%), dan F3 (2%). Evaluasi meliputi organoleptik, homogenitas, pH, viskositas, daya sebar, stabilitas busa, stabilitas cycling test, dan uji iritasi. Hasil menunjukkan seluruh formula memenuhi karakteristik fisik yang baik, stabil, dengan pH 4,9-6,2, viskositas 3.347-13.945 cPs, daya sebar 5,2-6,6 cm, dan stabilitas busa 62,04-68,00%. Tidak ditemukan tanda iritasi pada seluruh responden. Berdasarkan hasil penelitian, ekstrak etanol 80% daun sirih cina memiliki aktivitas antioksidan sangat kuat dan dapat diformulasikan menjadi sediaan facial wash gel yang aman digunakan.

Kata kunci: Daun Sirih cina, antioksidan, DPPH, facial wash, gel

Facial skin is the part of the body most frequently exposed to free radicals that can cause skin damage such as dullness, discoloration, and premature aging. Antioxidants play a role in counteracting free radicals by donating electrons, thereby inhibiting oxidative chain reactions. One of the plants that has the potential as a source of antioxidants is the Chinese betel leaf (Peperomia pellucida L. Kunth). This study aims to determine the antioxidant activity of 80% ethanol extract of Chinese betel leaves using the DPPH method and to evaluate the physical characteristics of facial wash gel preparations formulated from the extract. The simplicia was made by drying at room temperature for 30 days and extracted using the maceration method with 80% ethanol (1:10), resulting in an extract yield of 13.39%. Phytochemical screening showed the presence of alkaloids, flavonoids, saponins, tannins, triterpenoids, and steroids. Antioxidant test with DPPH method using UV-Vis spectrophotometer at 515 nm showed IC₅₀ value of 49.81 ppm (very strong category), while vitamin C as a positive control had IC₅₀ of 1.2 ppm. The extract was formulated into facial wash gel with various concentrations of F0 (0%), F1 (0.5%), F2 (1%), and F3 (2%). Evaluation included organoleptic, homogeneity, pH, viscosity, spreadability, foam stability, cycling test stability, and irritation test. The results showed that all formulas met good physical characteristics, were stable, with pH 4.9-6.2, viscosity 3,347-13,945 cPs, spreadability 5.2-6.6 cm, and foam stability 62.04-68.00%. No signs of irritation were found in all respondents. Based on the research results, 80% ethanol extract of Chinese betel leaves has very strong antioxidant activity and can be formulated into a safe facial wash gel preparation. Keywords: Chinese betel leaves, antioxidants, DPPH, facial wash, gel