Electronic Theses and Dissertation

Obesitas merupakan masalah kesehatan yang berkaitan dengan tingginya penyerapan lemak dalam tubuh. Salah satu strategi pengendaliannya adalah melalui penghambatan aktivitas enzim lipase pankreas. Aktinobakteri, khususnya genus Streptomyces, diketahui menghasilkan metabolit sekunder yang berpotensi sebagai inhibitor lipase. Penelitian ini bertujuan untuk mengetahui aktivitas variasi waktu inkubasi terhadap inhibitor lipase pankreas yang dihasilkan oleh isolat aktinobakteri ATS11 dan Streptomyces sp. ATS14 asal tanah Stasiun Penelitian Soraya, Kawasan Ekosistem Leuser Aceh, serta membandingkan aktivitas inhibitor lipase antara supernatan dan ekstrak metanol kedua isolat. Penelitian dilakukan secara eksperimental dengan variasi waktu inkubasi 5, 10, 15, dan 20 hari. Aktivitas inhibitor lipase ditentukan berdasarkan persentase penghambatan terhadap enzim lipase pankreas menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa aktivitas inhibitor lipase pada isolat ATS11 dan Streptomyces sp. ATS14 memiliki nilai yang berbeda pada setiap variasi waktu inkubasi. Aktivitas inhibisi tertinggi diperoleh pada waktu inkubasi 10 hari, yaitu sebesar 80,00% pada ATS11 dan 95,00% pada Streptomyces sp. ATS14. Ekstrak metanol ATS11 dan Streptomyces sp. ATS14 menunjukkan aktivitas inhibisi masing-masing sebesar 83,74% dan 77,16%. Berdasarkan hasil tersebut, kedua isolat berpotensi sebagai sumber inhibitor lipase pankreas, dengan aktivitas inhibisi tertinggi diperoleh pada waktu inkubasi 10 hari.
Kata kunci: Aktinobakteri, Streptomyces sp., Inhibitor Lipase Pankreas, Metabolit
Sekunder, Obesitas.

Obesity is a global health problem associated with excessive fat absorption in the body. One of the strategies for managing obesity is the inhibition of pancreatic lipase activity, an enzyme responsible for the hydrolysis of dietary triglycerides. Actinobacteria, particularly the genus Streptomyces, are known to produce secondary metabolites with potential lipase inhibitory activity. This study aimed to evaluate the effect of incubation time on the pancreatic lipase inhibitory activity of actinobacterial isolate ATS11 and Streptomyces sp. ATS14 isolated from the soil of the Soraya Research Station, Leuser Ecosystem, Aceh, and to compare the lipase inhibitory activity of their culture supernatants and methanolic extracts. The study was conducted experimentally using incubation periods of 5, 10, 15, and 20 days. Lipase inhibitory activity was determined based on the percentage of inhibition against pancreatic lipase using a UV-Vis spectrophotometer. The results showed that the lipase inhibitory activity of isolates ATS11 and Streptomyces sp. ATS14 varied across different incubation periods. The highest inhibitory activity was observed after 10 days of incubation, reaching 80.00% for ATS11 and 95.00% for Streptomyces sp. ATS14. The methanolic extracts of ATS11 and Streptomyces sp. ATS14 exhibited lipase inhibitory activities of 83.74% and 77.16%, respectively. Based on these findings, both isolates have the potential to serve as sources of pancreatic lipase inhibitors, with the highest inhibitory activity observed after 10 days of incubation. Keywords: Actinobacteria, Streptomyces sp., Pancreatic Lipase Inhibitor, Obesity.