Electronic Theses and Dissertation

Resistensi antibiotik pada bakteri patogen menjadi permasalahan penting dalam biandg kesehatan hewan and kesehatan masyarakat. Penelitian ini bertujuan untuk mengevaluasi aktivitas antibakteri ekstrak kapang halotoleran Aspergillus westerdijkiae B.K 9.2 terhadap Staphylococcus aureus serta menentukan nilai Minimum Inhibitory Concentration (MIC). Aktivitas antibakteri diuji menggunakan metode difusi cakram Kirby–Bauer, seandgkan nilai MIC ditentukan menggunakan metode mikrodilusi berbasis indikator resazurin. Hasil skrining awal menggunakan metode difusi cakram tanpa penentuan konsentrasi menunjukkan aandya aktivitas antibakteri ekstrak terhadap Staphylococcus aureus. Pengujian lanjutan menggunakan ekstrak pada konsentrasi 5000 ppm menghasilkan zona hambat rata-rata sebesar 7,5 ± 0,71 mm terhadap Staphylococcus aureus. Nilai MIC ekstrak terhadap Staphylococcus aureus sebesar 5000 ppm, dengan perbandingan kontrol positif ampisilin menunjukkan nilai MIC sebesar 625 ppm. Berdasarkan hasil penelitian, dapat disimpulkan bahwa ekstrak kapang halotoleran Aspergillus westerdijkiae B.K 9.2 memiliki aktivitas antibakteri terhadap Staphylococcus aureus and berpotensi sebagai sumber senyawa antibakteri untuk penelitian selanjutnya.

Antibiotic resistance among pathogenic bacteria has become a major challenge in both veterinary and public health. This study aimed to evaluate the antibacterial activity of the halotolerant fungus Aspergillus westerdijkiae B.K 9.2 extract against Staphylococcus aureus and to determine its minimum inhibitory concentration (MIC). Antibacterial activity was evaluated using the Kirby–Bauer disk diffusion method, while the MIC was determined using the resazurin-based broth microdilution method. Preliminary screening using the disk diffusion assay without concentration standardization demonstrated antibacterial activity against Staphylococcus aureus. Subsequent testing using an extract concentration of 5000 ppm produced an average inhibition zone of 7.5 ± 0.71 mm. The MIC of the extract against Staphylococcus aureus was determined to be 5000 ppm, whereas the positive control, ampicillin, exhibited an MIC of 625 ppm. These findings indicate that the extract of the halotolerant fungus Aspergillus westerdijkiae B.K 9.2 possesses antibacterial activity against Staphylococcus aureus. Nevertheless, the extract demonstrates potential as a natural source of antibacterial compounds for further research.