Electronic Theses and Dissertation

Bobot badan, konsentrasi testosteron seminal plasma, dan volume semen merupakan indikator penting yang menentukan kualitas fungsi reproduksi pejantan sapi aceh. Penelitian ini bertujuan untuk mengetahui hubungan antara bobot badan, konsentrasi testosteron seminal plasma, dan volume semen pada pejantan sapi aceh. Sebanyak lima ekor sapi aceh jantan yang sehat dengan bobot badan berkisar antara 248–278 kg digunakan dalam penelitian ini. Semen dikoleksi menggunakan vagina buatan, dan volume semen dicatat segera setelah koleksi. Seminal plasma dipisahkan dengan sentrifugasi pada kecepatan 5000 rpm selama 10 menit, dan konsentrasi testosteron diukur menggunakan metode enzyme-linked immunosorbent assay (ELISA). Analisis korelasi Pearson dilakukan untuk mengevaluasi hubungan antara bobot badan, konsentrasi testosteron seminal plasma dan volume semen. Hasil penelitian menunjukkan bahwa rata-rata bobot badan, konsentrasi testosteron seminal plasma, dan volume semen berturut-turut adalah 259 ± 11,6 kg, 4,29 ± 2,37 ng/mL, dan 4,7 ± 1,6 mL. Analisis korelasi Pearson menunjukkan korelasi sedang antara bobot badan dengan konsentrasi testosteron (r = 0,415; p = 0,487), maupun antara bobot badan dengan volume semen (r = 0,476; p = 0,417). Korelasi lemah ditemukan antara konsentrasi testosteron dengan volume semen (r = 0,326; p = 0,592). Kesimpulannya, bobot badan memiliki korelasi positif menengah yang tidak signifikan dengan konsentrasi testosteron seminal plasma dan volume semen, sedangkan volume semen memiliki korelasi positif lemah yang tidak signifikan dengan kadar testosteron seminal plasma pada pejantan sapi aceh.

Body weight, seminal plasma testosterone concentration, and semen volume are important indicators determining the quality of reproductive function of Aceh cattle bulls. This study aimed to determine the relationship between body weight, seminal plasma testosterone concentration, and semen volume in aceh bulls. A total of five healthy aceh bulls with body weights ranging from 248–278 kg were used in this study. Semen was collected using an artificial vagina, and semen volume was recorded immediately after collection. Seminal plasma was separated by centrifugation at 5000 rpm for 10 minutes, and testosterone concentration was measured using the enzyme-linked immunosorbent assay (ELISA) method. Pearson correlation analysis was performed to evaluate the relationship between body weight, seminal plasma testosterone concentration, and semen volume. The results showed that the average body weight, seminal plasma testosterone concentration, and semen volume were 259 ± 11.6 kg, 4.29 ± 2.37 ng/mL, and 4.7 ± 1.6 mL, respectively. Pearson correlation analysis showed a moderate correlation between body weight and testosterone concentration (r = 0.415; p = 0.487), as well as between body weight and semen volume (r = 0.476; p = 0.417). A weak correlation was found between testosterone concentration and semen volume (r = 0.326; p = 0.592). In conclusion, body weight had a non-significant positive moderate correlation with seminal plasma testosterone concentration and semen volume, while semen volume had a non-significant positive weak correlation with seminal plasma testosterone levels in aceh bulls.