Electronic Theses and Dissertation

Ginjal memiliki kerentanan tinggi terhadap paparan zat toksik akibat
tingginya aliran darah, sehingga berpotensi mengalami perubahan struktur akibat
pemberian senyawa bioaktif dalam jangka waktu panjang. Penelitian ini bertujuan
untuk mengevaluasi efek pemberian ekstrak etanol daun kari (Murraya koenigii)
selama 60 hari terhadap gambaran histopatologi ginjal pada tikus putih (Sprague
Dawley) model tumor kulit. Penelitian ini menggunakan desain observasional
dengan tiga kelompok, yaitu kontrol (P0), T1 (100 mg/kg BB), dan T2 (200
mg/kg BB). Ekstrak diberikan peroral selama 60 hari. Pada hari ke-61 tikus
dieuthanasia lalu organ ginjal dikoleksi, diproses dengan pewarnaan hematoksilin
eosin, dan diamati secara histopatologis berdasarkan parameter degenerasi sel,
kongesti, infiltrasi sel radang, dan nekrosis menggunakan sistem skoring. Hasil
penelitian menunjukkan bahwa kelompok kontrol menunjukkan struktur ginjal
normal. Kelompok T1 mengalami perubahan berupa nekrosis dan hemoragi,
sedangkan kelompok T2 menunjukkan kerusakan yang lebih berat meliputi
degenerasi sel, infiltrasi sel inflamasi, hemoragi, dan nekrosis. Disimpulkan
bahwa pemberian ekstrak etanol daun kari selama 60 hari berpotensi
menyebabkan kerusakan histopatologi ginjal dengan derajat keparahan yang
meningkat seiring peningkatan dosis. Temuan ini menunjukkan perlunya kajian
lebih lanjut untuk menentukan batas aman penggunaan ekstrak daun kari sebagai
terapi herbal.

The kidney has a high susceptibility to toxic substances due to its substantial blood flow, making it prone to structural alterations following long term administration of bioactive compounds. This study aimed to evaluate the effects of ethanolic extract of curry leaves (Murraya koenigii) administered for 60 days on the histopathological features of the kidneys in white rats (Sprague Dawley) with a skin tumor model. This study employed an observational design with three groups: control (P0), T1 (100 mg/kg body weight), and T2 (200 mg/kg body weight). The extract was administered orally for 60 days. On day 61, the rats were euthanized, and kidney organs were collected, processed using hematoxylin eosin staining, and examined histopathologically based on parameters including cellular degeneration, congestion, inflammatory cell infiltration, and necrosis using a scoring system. The results showed that the control group exhibited normal kidney structure. The T1 group demonstrated changes in the form of necrosis and hemorrhage, while the T2 group exhibited more severe damage, including cellular degeneration, inflammatory cell infiltration, hemorrhage, and necrosis. It can be concluded that the administration of ethanolic extract of curry leaves for 60 days has the potential to induce histopathological kidney damage with increasing severity corresponding to higher doses. These findings indicate the need for further studies to determine the safe limits of curry leaf extract use as an herbal therapy.