Electronic Theses and Dissertation

Daging ayam adalah sumber protein yang sangat disukai oleh masyarakat Indonesia.
Menurut data Survei Sosial Ekonomi Nasional, konsumsi daging ayam ras pedaging per kapita terus
bertambah setiap tahunnya sebesar 7,87%. Penelitian ini bertujuan untuk mengisolasi dan
mengidentifikasi Salmonella sp. pada daging ayam di Pasar Tradisional Banda Aceh. Penelitian ini
merupakan metode observasional laboratorium dengan menggunakan analisis deskriptif. Sampel
daging ayam diambil dari lima pasar tradisional Banda Aceh. Daging ayam yang diduga tercemar
Salmonella sp. diisolasi ke dalam media pra-pengkayaan yaitu Buffer Peptone Water (BPW) dan
diinkubasi selama 24 jam. Selanjutnya kultur dipindahkan ke dalam media pengkayaan yaitu
Selenite Cystine Broth (SCB) dan diinkubasi selama 18 jam, kemudian dilakukan kembali
penanaman pada media spesifik Salmonella Shigella Agar (SSA) dan diinkubasi selama 24 jam.
Setelah diinkubasi diperoleh 23 koloni yang diduga Salmonella sp. dari 30 sampel yang diperiksa.
Kemudian dari koloni tersebut setelah dilakukan uji biokimia menunjukkan negatif Salmonella sp.
tetapi dideteksi terdapat bakteri lain seperti dua koloni Klebsiella pneumoniae, tiga koloni Proteus
vulgaris, dua koloni P. mirabillis, tiga koloni Escherichia coli, dan satu koloni Shigella sp.. Dengan
demikian dapat disimpulkan bahwa Salmonella pada penelitian ini tidak ditemukan pada daging
ayam yang dijual di pasar tradisional Banda Aceh, tetapi bakteri lain terdeteksi seperti Klebsiella
pneumoniae, Proteus vulgaris, P. mirabillis, Escherichia coli, Shigella sp.

Chicken meat is a highly favored source of protein by the Indonesian people. According to the National Socioeconomic Survey data, per capita consumption of broiler chicken meat continues to increase annually by 7.87%. This study aims to isolate and identify Salmonella sp. in chicken meat in Banda Aceh Traditional Markets. This study is an observational laboratory method using descriptive analysis. Chicken meat samples were taken from five traditional markets in Banda Aceh. Chicken meat suspected of being contaminated with Salmonella sp. was isolated into a preenrichment medium, namely Buffer Peptone Water (BPW), and incubated for 24 hours. Next, the culture was transferred into an enrichment medium, namely Selenite Cystine Broth (SCB), and incubated for 18 hours. Then, it was re-planted on a specific Salmonella Shigella Agar (SSA) medium and incubated for 24 hours. After incubation, 23 colonies suspected of being Salmonella sp. were obtained from 30 samples examined. Then, from these colonies, biochemical tests showed negative Salmonella sp. but other bacteria were detected such as two colonies of Klebsiella pneumoniae, three colonies of Proteus vulgaris, two colonies of P. mirabillis, three colonies of Escherichia coli, and one colony of Shigella sp.. Thus it can be concluded that Salmonella in this study was not found in chicken meat sold in the traditional market of Banda Aceh, but other bacteria were detected such as Klebsiella pneumoniae, Proteus vulgaris, P. mirabillis, Escherichia coli, Shigella sp.