Electronic Theses and Dissertation

Striae distensae merupakan kelainan kulit yang ditandai dengan kerusakan matriks ekstraseluler akibat peningkatan degradasi kolagen dan elastin serta stres oksidatif. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak etanol Centella asiatica menggunakan metode 2,2-diphenyl-1-picrylhydrazyl (DPPH) serta menganalisis interaksi molekuler senyawa aktifnya terhadap protein target yang berperan dalam pembentukan striae secara in silico. Penelitian meliputi ekstraksi menggunakan etanol 70%, pengujian aktivitas antioksidan metode DPPH, dan studi molecular docking terhadap protein target Matrix Metalloproteinase-1 (MMP-1), Matrix Metalloproteinase-13 (MMP-13), elastase, kolagen, dan Transforming Growth Factor-Beta (TGF-β) receptor. Senyawa aktif yang dianalisis meliputi asiaticoside, madecassoside, asiatic acid, dan madecassic acid. Hasil penelitian menunjukkan bahwa ekstrak etanol Centella asiatica memiliki aktivitas antioksidan kuat dengan nilai IC₅₀ sebesar 55,46 ppm. Hasil molecular docking menunjukkan bahwa asiaticoside memiliki nilai binding affinity sebesar -6,9; -7,7; -7,4; -5,1; dan -6,8 kkal/mol terhadap MMP-1, MMP-13, elastase, kolagen, dan TGF-β receptor. Madecassoside menunjukkan nilai binding affinity sebesar -6,7; -7,9; -7,7; -4,7; dan -7,0 kkal/mol terhadap kelima protein target tersebut. Asiatic acid memiliki nilai binding affinity sebesar -6,8; -7,6; -7,0; -4,9; dan -6,4 kkal/mol, sedangkan madecassic acid menunjukkan nilai binding affinity sebesar -6,7; -7,5; -7,1; -4,8; dan -6,5 kkal/mol terhadap MMP-1, MMP-13, elastase, kolagen, dan TGF-β receptor secara berturut-turut. Nilai binding affinity yang negatif serta terbentuknya ikatan hidrogen dan interaksi hidrofobik menunjukkan kestabilan kompleks ligan-protein yang terbentuk. Berdasarkan hasil tersebut, ekstrak etanol Centella asiatica berpotensi dikembangkan sebagai agen anti-striae melalui aktivitas antioksidan, penghambatan degradasi matriks ekstraseluler, serta dukungan terhadap regenerasi jaringan kulit.
Kata kunci: Centella asiatica, striae distensae, antioksidan, DPPH, molecular docking.

Striae distensae is a skin disorder characterized by damage to the extracellular matrix due to increased collagen and elastin degradation as well as oxidative stress. This study aimed to evaluate the antioxidant activity of Centella asiatica ethanolic extract using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and to analyze the molecular interactions of its bioactive compounds with protein targets involved in the pathogenesis of striae through an in silico approach. The study involved extraction using 70% ethanol, antioxidant activity testing by the DPPH method, and molecular docking analysis against Matrix Metalloproteinase-1 (MMP-1), Matrix Metalloproteinase-13 (MMP-13), elastase, collagen, and Transforming Growth Factor-Beta (TGF-β) receptor. The bioactive compounds evaluated were asiaticoside, madecassoside, asiatic acid, and madecassic acid. The results showed that Centella asiatica ethanolic extract exhibited strong antioxidant activity with an IC₅₀ value of 55.46 ppm. Molecular docking analysis revealed that asiaticoside demonstrated binding affinity values of -6.9, -7.7, -7.4, -5.1, and -6.8 kcal/mol against MMP-1, MMP-13, elastase, collagen, and TGF-β receptor, respectively. Madecassoside showed binding affinity values of -6.7, -7.9, -7.7, -4.7, and -7.0 kcal/mol against the respective protein targets. Asiatic acid exhibited binding affinity values of -6.8, -7.6, -7.0, -4.9, and -6.4 kcal/mol, while madecassic acid demonstrated binding affinity values of -6.7, -7.5, -7.1, -4.8, and -6.5 kcal/mol against MMP-1, MMP-13, elastase, collagen, and TGF-β receptor, respectively. The negative binding affinity values, along with the formation of hydrogen bonds and hydrophobic interactions, indicate the stability of the ligand–protein complexes formed. These findings suggest that Centella asiatica ethanolic extract has potential as an anti-striae agent through its antioxidant activity, inhibition of extracellular matrix degradation, and support of skin tissue regeneration. Keywords: Centella asiatica, striae distensae, antioxidant, DPPH, molecular docking.