Electronic Theses and Dissertation

Penelitian ini bertujuan untuk mengetahui efektivitas masker peel-off kombinasi ekstrak daun salam (Syzygium polyanthum) dan Virgin Coconut Oil (VCO) sebagai antibakteri dan antioksidan alami. Formulasi masker peel-off dibuat dalam empat variasi konsentrasi, yaitu MP 0%, MP 5%, MP 10% dan MP 15%. Analisis GC-MS dilakukan menggunakan instrument Gas Chromatography – Mass Spectroscopy. Uji aktivitas antibakteri terhadap Staphylococcus aureus dan Propionibacterium acnes menggunakan metode difusi cakram. Uji aktivitas antioksidan dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Uji organoleptik dengan pengisian kuisioner oleh 30 responden. Hasil uji GC-MS menunjukkan terdapat 41 senyawa kimia pada ekstrak daun salam dan 13 senyawa kimia pada VCO. Uji antibakteri menunjukkan bahwa ekstrak daun salam memiliki aktivitas antibakteri kategori sedang terhadap Staphylococcus aureus dan lemah terhadap Propionibacterium acnes. VCO memiliki aktivitas antibakteri kategori lemah terhadap kedua bakteri. Sediaan masker peel-off memiliki aktivitas antibakteri kategori kuat terhadap Staphylococcus aureus pada konsentrasi 10% dan 15%, sedangkan terhadap Propionibacterium acnes aktivitasnya tergolong lemah. Hasil aktivitas antioksidan menunjukkan bahwa ekstrak daun salam memiliki aktivitas antioksidan sangat kuat. Aktivitas antioksidan pada VCO dikategorikan lemah. Aktivitas antioksidan sediaan masker peel-off pada konsentrasi 15% dikategorikan sangat kuat. Formula masker MP 5% paling disukai panelis karena memiliki warna hijau pekat kekuningan, sedikit beraroma herbal, dan tekstur kental mudah diaplikasikan. Kombinasi ekstrak daun salam dan VCO berpotensi sebagai bahan aktif alami dalam sediaan masker peel-off dengan efektivitas tinggi sebagai antioksidan dan antibakteri terhadap Staphylococcus aureus.

Kata kunci: Antioksidan, Antibakteri, Daun Salam, Masker peel-off, VCO

This study aims to determine the effectiveness of a peel-off mask combining bay leaf extract (Syzygium polyanthum) and Virgin Coconut Oil (VCO) as a natural antibacterial and antioxidant. The peel-off mask formulation was made in four concentration variations, namely MP 0%, MP 5%, MP 10% and MP 15%. GC-MS analysis was carried out using a Gas Chromatography – Mass Spectroscopy instrument. Antibacterial activity tests against Staphylococcus aureus and Propionibacterium acnes used the disc diffusion method. Antioxidant activity tests used the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Organoleptic tests by filling out questionnaires by 30 respondents. The results of the GC-MS test showed that there were 41 chemical compounds in bay leaf extract and 13 chemical compounds in VCO. The antibacterial test showed that bay leaf extract had moderate antibacterial activity against Staphylococcus aureus and weak against Propionibacterium acnes. VCO had weak antibacterial activity against both bacteria. The peel-off mask preparation had strong antibacterial activity against Staphylococcus aureus at concentrations of 10% and 15%, while its activity was weak against Propionibacterium acnes. The antioxidant activity results showed that bay leaf extract had very strong antioxidant activity. The antioxidant activity of VCO was categorized as weak. The antioxidant activity of the peel-off mask preparation at a concentration of 15% was categorized as very strong. The 5% MP mask formula was the most preferred by panelists due to its deep yellowish-green color, slight herbal aroma, and thick texture that was easy to apply. The combination of bay leaf extract and VCO has the potential to be a natural active ingredient in peel-off masks with high antioxidant and antibacterial effectiveness against Staphylococcus aureus. Keywords: Antioxidant, Antibacterial, Bay Leaf, Peel-off Mask, VCO