Electronic Theses and Dissertation

Daun kari (Murraya koenigii) merupakan salah satu tanaman herbal yang berkhasiat dalam
pengobatan tumor. Penggunaan herbal dalam jangka panjang berpotensi menimbulkan efek
samping, pada organ pencernaan sebagai tempat absorpsi utama. Penelitian ini bertujuan untuk
mengetahui gambaran histopatologi usus halus tikus Sprague Dawley model tumor kulit setelah
pemberian ekstrak etanol daun kari selama 60 hari. Penelitian ini merupakan penelitian
eksperimental laboratorium dengan rancangan acak lengkap menggunakan 9 ekor tikus Sprague
Dawley jantan model tumor kulit. Tikus dibagi menjadi tiga kelompok, yaitu kelompok kontrol
tumor kulit (P0), kelompok perlakuan tikus model tumor kulit yang diberikan ekstrak etanol daun
kari dosis 100 dan 200 mg/kg BB (P1 dan P2) diberikan secara oral selama 60 hari. Tikus
dieutanasi pada hari ke-61 dan organ usus halus dikoleksi, dilanjutkan dengan pembuatan preparat
histopatologi dengan pewarnaan hematoksilin dan eosin. Parameter pengamatan berupa
deskuamasi epitel, infiltrasi sel radang, proliferasi sel radang, hemoragi dan kongesti berdasarkan
skoring ringan, sedang dan berat. Hasil pengamatan pada kelompok P0 memperlihatkan struktur
jaringan usus halus dalam keadaan normal. Kelompok P1 memperlihatkan deskuamasi epitel, infiltrasi sel radang, proliferasi sel goblet serta hemoragi. Kelompok P2 menunjukkan perubahan
histopatologi yang disertai adanya kongesti. Kelompok P1 memperlihatkan kerusakan dengan
kategori ringan hingga sedang sedangkan kelompok P2 memperlihatkan kerusakan dengan
kategori ringan hingga berat. Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian
ekstrak etanol daun kari selama 60 hari memengaruhi struktur histopatologi usus halus tikus
Sprague Dawley model tumor kulit, dengan perubahan yang meningkat seiring kenaikan dosis.

Curry leaves (Murraya koenigii) are a medicinal plant known for various therapeutic properties, including potential use in tumor treatment; however, long-term use of herbal remedies may lead to side effects, particularly in the digestive organs, which serve as the primary site of absorption. This study aimed to evaluate the histopathological features of the small intestine in Sprague Dawley rats with a skin tumor model after administration of ethanol extract of curry leaves for 60 days. This research was an experimental laboratory study using a completely randomized design with nine male Sprague Dawley rats bearing a skin tumor model, divided into three groups: the control group with skin tumors (P0) and two treatment groups receiving ethanol extract of curry leaves at doses of 100 mg/kg body weight (P1) and 200 mg/kg body weight (P2), administered orally for 60 days. On day 61, the rats were euthanized, and the small intestines were collected for histopathological preparation using hematoxylin and eosin staining. The observed parameters included epithelial desquamation, inflammatory cell infiltration, inflammatory cell proliferation, hemorrhage, and congestion, which were assessed using a scoring system categorized as mild, moderate, or severe. The results showed that the P0 group exhibited normal small intestinal histological structure, while the P1 group showed epithelial desquamation, inflammatory cell infiltration, goblet cell proliferation, and hemorrhage, and the P2 group demonstrated similar histopathological changes along with congestion; the P1 group exhibited mild to moderate damage, whereas the P2 group showed mild to severe damage. In conclusion, administration of ethanol extract of curry leaves for 60 days affects the histopathological structure of the small intestine in Sprague Dawley rats with a skin tumor model, with more severe changes observed at higher doses.