Electronic Theses and Dissertation

Kualitas semen pada domba dipengaruhi oleh berbagai faktor, termasuk status
mikromineral yang berperan penting dalam fungsi reproduksi. Namun, data terkait perbedaan
konsentrasi mikromineral antar breed dan hubungannya dengan kualitas semen masih terbatas.
Penelitian ini bertujuan untuk mengevaluasi perbedaan konsentrasi mikromineral (Zn, Fe, Mn, dan
Co) antara dua breed domba, yaitu domba komposit sumatera dan domba garut, serta hubungannya
dengan kualitas semen. Penelitian ini menggunakan 10 ekor pejantan (masing-masing 5 ekor)
berumur sekitar tiga tahun yang dipelihara dengan manajemen dan pakan yang sama. Sampel semen
dan plasma darah diambil dan dianalisis terhadap parameter konsentrasi, motilitas, dan viabilitas
sperma serta kadar mikromineral menggunakan Atomic Absorption Spectrophotometer(AAS). Hasil
penelitian menunjukkan bahwa kadar Fe berbeda signifikan antara kedua breed (P0,05). Uji korelasi Pearson menunjukkan bahwa Fe dan
Zn berkorelasi negatif signifikan terhadap motilitas (r = -0,782 dan r = -0,715) dan Zn juga terhadap
viabilitassperma (r = -0,726). Tidak terdapat korelasi signifikan antara Co dan Mn dengan parameter
kualitas semen. Hasil penelitian ini menunjukkan bahwa kadar mikromineral, khususnya Fe dan Zn,
dapat memengaruhi motilitas dan viabilitas sperma, sehingga berpotensi dijadikan sebagai
biomarker kualitas semen pada domba komposit sumatera dan domba garut.

Semen quality in rams is influenced by various factors, including micromineral status, which plays a crucial role in reproductive function. However, data on differences in micromineral concentrations between breeds and their relationship with semen quality remain limited. This study aimed to evaluate the differences in micromineral concentrations (Zn, Fe, Mn, and Co) between two sheep breeds, namely the Sumatera composite sheep and the Garut sheep, as well as their association with semen quality. The study used 10 rams (five from each breed), approximately three years old, maintained underthe same management and feeding conditions. Semen and blood plasma samples were collected and analyzed for sperm concentration, motility, and viability parameters, along with micromineral levels using Atomic Absorption Spectrophotometry (AAS). The results showed that Fe levels differed significantly between the two breeds (P0.05). Pearson correlation analysis revealed a significant negative correlation between Fe and sperm motility (r = -0.782), as well as between Zn and sperm motility (r = -0.715) and viability (r = - 0.726). No significant correlations were observed between Co and Mn and any of the semen quality parameters. These findings suggest that micromineral levels, particularly Fe and Zn, may influence sperm motility and viability, indicating their potential as biomarkers of semen quality in Sumatera composite and Garut sheep.