Electronic Theses and Dissertation

Latar Belakang: Madu telah mulai banyak digunakan sebagai terapi penyembuhan luka di berbagai belahan dunia, termasuk Aceh, Indonesia, karena sifat antibakteri dan antiinflamasinya. Namun, masih sedikit penelitian mengenai formulasi bahan madu dalam bentuk modern dressing yang tersedia.
Tujuan Penelitian: Menilai pengaruh hydrocolloid dressing berbahan dasar madu kelulut Aceh terhadap penyembuhan luka secara makroskopis dan mikroskopis.
Metode Penelitian: 30 ekor mencit jantan dewasa yang sehat, berusia 2-3 bulan, diberikan dua luka terbuka di punggungnya dengan diameter 4 mm. Mencit kemudian dibagi menjadi tiga kelompok: satu kelompok menerima normal saline tanpa madu kelulut Aceh (K), satu kelompok menerima madu kelulut Aceh murni (MK) dan satu kelompok menerima hydrocolloid dressing berbahan dasar madu kelulut Aceh (HC). Diameter luka dihitung setiap hari hingga hari ke-14. Biopsi kulit diambil dan diperiksa secara histopatologi pada hari ke-3, 7, dan 14 setelah perlakuan. Penilaian histologis penyembuhan luka didasarkan pada jumlah neutrofil, makrofag, rasio epitelisasi, miofibroblas, dan kepadatan serat kolagen. Data dianalisis menggunakan uji statistik ANOVA atau Kruskal-Wallis, dilanjutkan dengan uji Mann Whitney.
Hasil Penelitian: Hasil uji statistik menunjukkan terdapat perbedaan yang signifikan pada jumlah neutrofil, rasio epitelisasi, rasio miofibroblas, dan rasio kepadatan serat kolagen antara kelompok HC, MK dengan kelompok K (p

Background: Honey has been widely used as a wound healing therapy in various parts of the world, including Aceh, Indonesia, due to its antibacterial and antiinflammatory properties. However, limited research on the formulation of honey ingredients in the form of modern dressings is available. Objective: To assess the effect of Aceh kelulut honey-based hydrocolloid dressing on wound healing macroscopically and microscopically. Methods: 30 healthy adult male mice, 2-3 months old, were given two open wounds on their backs with a diameter of 4 mm. The mice were then divided into three groups: one group received normal saline without Aceh kelulut honey (K), one group received pure Aceh kelulut honey (MK) and one group received Aceh kelulut honey-based hydrocolloid dressing (HC). Wound diameter was calculated daily until day 14. Skin biopsies were taken and examined histopathologically on days 3, 7, and 14 after treatment. Histological assessment of wound healing was based on the number of neutrophils, macrophages, epithelialization ratio, myofibroblasts, and collagen fiber density. Data were analyzed using ANOVA or Kruskal-Wallis statistical test, followed by Mann Whitney test. Results: Statistical test results showed that there were significant differences in the number of neutrophils, epithelialization ratio, myofibroblast ratio, and collagen fiber density ratio between the HC, MK and K groups (p0.05). Conclusion: The application of Aceh kelulut honey-based hydrocolloid dressing to the wound can accelerate wound healing, reduce the number of neutrophils, increase the ratio of myofibroblasts, and increase the density ratio of collagen fibers, but inhibit the ratio of wound epithelialization and do not affect the number of macrophages in both the inflammatory phase and the proliferation phase. Keywords: hydrocolloid dressing, Aceh kelulut honey, neutrophil, macrophages, epithelialization