Electronic Theses and Dissertation

Peningkatan paparan stres oksidatif berperan penting dalam perkembangan berbagai penyakit degeneratif, sehingga diperlukan sumber antioksidan alami yang efektif dan aman. Penelitian ini bertujuan untuk menganalisis aktivitas antioksidan ekstrak biji saga serta mengevaluasi hubungannya dengan kandungan fenolik total secara in vitro menggunakan metode DPPH dan ABTS serta membandingkan efektivitas ekstraksi menggunakan pelarut etanol dan metanol 95% terhadap kemampuan penangkapan radikal bebas. Penelitian menggunakan rancangan eksperimental in vitro dengan pendekatan kuantitatif. Sampel biji saga diekstraksi melalui metode maserasi menggunakan pelarut etanol dan metanol 95%. Aktivitas antioksidan ditentukan berdasarkan persentase inhibisi dan nilai IC₅₀ menggunakan metode DPPH dan ABTS, sedangkan kandungan fenolik total dianalisis dengan metode Folin Ciocalteu. Hasil penelitian menunjukkan bahwa ekstrak biji saga memiliki aktivitas antioksidan yang kuat. Ekstrak metanol pada aktivitas antioksidan DPPH menunjukkan nilai IC₅₀ sebesar 86,43 ppm, sedangkan ekstrak etanol sebesar 99,11 ppm. Hasil pada uji ABTS ekstrak metanol nilai IC₅₀ 54,07 ppm, dan ekstrak etanol nilai IC₅₀ sebesar 72,41. Terdapat hubungan kuat antara aktivitas antioksidan dengan kandungan fenolik total. Hal ini mengindikasikan bahwa biji saga berpotensi dikembangkan sebagai sumber antioksidan alami yang aplikatif, terutama dalam bidang pangan fungsional dan farmasi.

Increased exposure to oxidative stress plays a significant role in the development of various degenerative diseases, thus necessitating the need for effective and safe natural antioxidant sources. This study aimed to analyze the antioxidant activity of saga seed extract and to evaluate its relationship with total phenolic content in vitro using the DPPH and ABTS methods, as well as to compare the effectiveness of extraction using 95% ethanol and methanol solvents on free radical scavenging capacity. The study employed an in vitro experimental design with a quantitative approach. Saga seed samples were extracted through maceration using 95% ethanol and methanol solvents. Antioxidant activity was determined based on percentage inhibition and IC₅₀ values using the DPPH and ABTS methods, while total phenolic content was analyzed using the Folin–Ciocalteu method. The results showed that saga seed extract exhibited strong antioxidant activity. The methanolic extract in the DPPH assay demonstrated an IC₅₀ value of 86.43 ppm, whereas the ethanolic extract showed an IC₅₀ value of 99.11 ppm. In the ABTS assay, the methanolic extract exhibited an IC₅₀ value of 54.07 ppm, while the ethanolic extract showed an IC₅₀ value of 72.41 ppm. A strong correlation was observed between antioxidant activity and total phenolic content. These findings indicate that saga seeds have potential to be developed as a practical natural antioxidant source, particularly in the fields of functional foods and pharmaceuticals.