Electronic Theses and Dissertation

Serai dapur (Cymbopogon citratus) merupakan tanaman obat yang diketahui mengandung senyawa metabolit sekunder yang berpotensi sebagai antiinflamasi. Salah satu mediator inflamasi yang berperan penting dalam respons inflamasi adalah interleukin-6 (IL-6). Penelitian ini bertujuan untuk menentukan potensi aktivitas antiinflamasi ekstrak etanol 80% Cymbopogon citratus dalam menekan produksi mediator inflamasi berupa IL-6 secara in vitro. Metode penelitian yang digunakan adalah metode eksperimental laboratorium menggunakan sel RAW 264.7 yang diinduksi lipopolisakarida (LPS). Pengukuran kadar IL-6 dilakukan menggunakan metode Enzyme-Linked Immunosorbent Assay (ELISA). Aktivitas antiinflamasi dinilai berdasarkan persentase inhibisi kadar IL-6 dan dianalisis secara statistik menggunakan uji Kruskal-Wallis (alternatif One Way ANOVA) yang dilanjutkan dengan uji Post Hoc Dunn dengan koreksi Bonferroni. Hasil penelitian menunjukkan bahwa ekstrak etanol 80% Cymbopogon citratus menghasilkan persentase inhibisi IL-6 yang tinggi pada seluruh konsentrasi uji. Namun, hasil uji statistik post hoc menunjukkan bahwa perbedaan yang signifikan secara statistik hanya terdapat pada pasangan kontrol sel normal dengan kontrol sel yang diinduksi LPS (p = 0,010) serta pada kelompok deksametason 4 ppm terhadap kontrol sel yang diinduksi LPS (p = 0,028). Kesimpulan penelitian ini menunjukkan bahwa ekstrak etanol 80% Cymbopogon citratus memiliki potensi aktivitas antiinflamasi secara biologis dalam menekan produksi IL-6, namun belum didukung oleh signifikansi statistik yang kuat.

Kata kunci: Cymbopogon citratus, antiinflamasi, interleukin-6, ELISA, sel RAW 264.7

Lemongrass (Cymbopogon citratus) is a medicinal plant known to contain secondary metabolites with potential anti-inflammatory activity. One of the inflammatory mediators that plays an important role in the inflammatory response is interleukin-6 (IL-6). This study aimed to determine the potential anti-inflammatory activity of the 80% ethanolic extract of Cymbopogon citratus in suppressing the production of the inflammatory mediator IL-6 in vitro. The research method used was an experimental laboratory method employing LPS-induced RAW 264.7 cells. Measurement of IL-6 levels was performed using the Enzyme-Linked Immunosorbent Assay (ELISA) method. Anti-inflammatory activity was assessed based on the percentage inhibition of IL-6 levels and statistically analyzed using the Kruskal–Wallis test (as an alternative to One-Way ANOVA), followed by the Post Hoc Dunn test with Bonferroni correction. The results showed that the 80% ethanolic extract of Cymbopogon citratus produced high percentages of IL-6 inhibition at all tested concentrations. However, the post hoc statistical test results showed that statistically significant differences were only found between the normal cell control and the LPS-induced cell control (p = 0.010) and between dexamethasone 4 ppm and the LPS-induced cell control (p = 0.028). The conclusion of this study indicates that the 80% ethanolic extract of Cymbopogon citratus has potential biological anti-inflammatory activity in suppressing IL-6 production, but this effect has not yet been supported by strong statistical significance. Keywords: Cymbopogon citratus, anti-inflammatory, interleukin-6, ELISA, RAW 264.7 cells