Electronic Theses and Dissertation

Diabetes mellitus tipe 2 (DMT2) adalah salah satu penyakit yang jika tidak segera ditangani dapat menyebabkan komplikasi seperti gangguan pada kualitas spermatozoa khususnya pergerakan dan jumlahnya. Kadar gula darah yang meningkat merupakan indikasi adanya DMT2 diperoleh melalui induksi streptozotocin (STZ). Penelitian ini bertujuan untuk mengetahui kualitas spermatozoa dan gambaran histopatologis testis mencit jantan setelah diberi ekstrak daun malaka (Phyllanthus emblica). Metode penelitian dilakukan secara ekperimental dengan menggunakan Rancangan Acak Lengkap (RAL). Mencit jantan (Mus musculus L.) yang digunakan sebanyak 25 ekor berusia ±1 bulan dengan berat badan ±20-40 gram dibagi menjadi lima kelompok yaitu K1 kontrol positif (aquadest), K2 kontrol negative (STZ 30mg/kgBB), P1 (STZ 30mg/kgBB dan ekstrak daun malaka 300mg/kgBB), P2 (STZ 30mg/kgBB dan ekstrak daun malaka 600mg/kgBB) dan P3 (STZ 30mg/kgBB dan ekstrak daun malaka 1200mg/kgBB). Perlakuan diberikan secara oral sekali sehari sebanyak 0,2 ml selama 14 hari. Variabel yang diamati meliputi motilitas, morfologi, viabilitas dan jumlah spermatozoa serta histopatologiss testis mencit. Data yang diperoleh dari pengamatan mikroskopis spermatozoa dianalisis dengan analysis of variance (ANOVA) pola satu arah untuk mengetahui perbedaan rata-rata yang diperoleh antar kelompok perlakuan kemudian dilanjutkan dengan uji Duncan dan pengamatan histopatologis dijelaskan secara deskriptif. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun malaka pada mencit jantan diabetes mellitus berpengaruh positif dalam meningkatkan viabilitas, motilitas, morfologi dan jumlah spermatozoa bertutut-turut yaitu pada mencit jantan (Mus musculus L.). Dibuktikan dengan pengamatan histopatologis testis mencit yang mengalami perbaikan yang signifikan setelah pemberian ekstrak daun malaka. Hasil penelitian dapat disimpulkan bahwa pemberian ekstrak daun malaka dapat meningkatkan kualitas spermatozoa dan mempengaruhi gambaran histopatologis dengan dosis 1200 mg/kg BB sebagai dosis terbaik untuk penyembuhan kualitas performa spermatozoa mencit jantan (DMT2).

Kata kunci: Diabetes mellitus, kualitas spermatozoa, histopatologis testis, ekstrak daun malaka

Type 2 diabetes mellitus (T2DM) is a disease that if not treated immediately can cause complications such as disorders in the quality of spermatozoa, especially their movement and quantity. Increased blood sugar levels are an indication of T2DM obtained through streptozotocin (STZ) induction. This study aims to determine the quality of spermatozoa and histopathological description of the testes of male mice after being given malacca leaf extract (Phyllanthus emblica). The research method was carried out experimentally using a Completely Randomized Design (CRD). Male mice (Mus musculus L.) used were 25, aged ±1 month with a body weight of ±20-40 grams, divided into five groups, namely K1 positive control (aquadest), K2 negative control (STZ 30mg/kgBW), P1 (STZ 30mg/kgBW and malacca leaf extract 300mg/kgBW), P2 (STZ 30mg/kgBW and malacca leaf extract 600mg/kgBW) and P3 (STZ 30mg/kgBW and malacca leaf extract 1200mg/kgBW). The treatment was given orally once a day as much as 0.2 ml for 14 days. The variables observed included motility, morphology, viability and number of spermatozoa and histopathology of the mice testes. Data obtained from microscopic observations of spermatozoa were analyzed by one-way analysis of variance (ANOVA) to determine the average differences obtained between treatment groups, then continued with the Duncan test and histopathological observations were explained descriptively. The results of the study showed that the administration of malacca leaf extract to male mice with diabetes mellitus had a positive effect on increasing viability, motility, morphology and number of spermatozoa in sequence, namely in male mice (Mus musculus L.). Proven by histopathological observations of mouse testes which experienced significant improvement after administration of malacca leaf extract. The results of the study can be concluded that administration of malacca leaf extract can improve spermatozoa quality and affect histopathological images with a dose of 1200 mg/kg BW as the best dose for healing the quality of spermatozoa performance in male mice (DMT2). Keywords: Diabetes mellitus, sperm quality, testicular histopathology, malacca leaf extract