Electronic Theses and Dissertation

Kriopreservasi sperma ikan betok (Anabas testudineus) telah dikembangkan oleh beberapa peneliti. Namun demikian, kualitas sperma pascakriopreservasi yang dihasilkan masih belum optimal dan berpotensi untuk ditingkatkan melalui optimasi teknik pra-pembekuan dan thawing. Oleh karena itu, penelitian ini bertujuan untuk menentukan teknik pra-pembekuan dan thawing terbaik dalam proses kriopreservasi sperma ikan betok. Penelitian ini dilaksanakan pada bulan Maret hingga April 2025 di Laboratorium Pembenihan dan Pembiakan Ikan, Fakultas Kelautan dan Perikanan, Universitas Syiah Kuala. Penelitian menggunakan Rancangan Acak Lengkap (RAL) nonfaktorial dan dilaksanakan dalam tiga tahap. Tahap pertama merupakan pencucian telur ikan betok dalam larutan EM-4, dengan 5 perlakuan yaitu EM-4 dosis 0 mg/L (control), 0.5 ml/L, 1.0 ml/L, 1.5 ml/L, and 2.0 ml/L. Tahap kedua merupakan pengujian beberapa teknik pra-pembekuan dengan gradien suhu yang berbeda, yaitu: F1 (4°C, 0°C, −79°C, dan −196°C), F2 (4°C, 0°C, dan −196°C), F3 (4°C, −79°C, dan −196°C), serta F4 (4°C dan −196°C). Tahap ketiga adalah pengujian teknik thawing dengan variasi suhu dan waktu thawing, yaitu pada suhu 25°C, 30°C, dan 35°C, masing-masing dengan lama perendaman 5, 10, dan 15 menit. Hasil percobaan pertama di dapatkan bahwa perendama telur dalam EM-4 dengan dosis 1,0 ml/L Adalah yang terbaik, Hasil percobaan tahap kedua menunjukkan bahwa suhu pra-pembekuan berpengaruh nyata terhadap motilitas, viabilitas, abnormalitas sperma, dan fertilitas telur ikan betok (P0,05). Kualitas sperma terbaik pascakriopreservasi diperoleh pada perlakuan F1, yaitu teknik pra-pembekuan dengan gradien suhu 4°C, 0°C, −79°C, dan −196°C, yang menghasilkan motilitas sperma sebesar 78,50 ± 1,70%, viabilitas 77,50 ± 1,91%, abnormalitas 1,50 ± 0,57%, fertilitas 62,50 ± 8,18%, serta masa hidup sperma 9,82 ± 1,58 menit. Pada percobaan tahap ketiga, hasil menunjukkan bahwa teknik thawing berpengaruh signifikan terhadap motilitas, viabilitas, abnormalitas, fertilitas, dan masa hidup sperma pascakriopreservasi (P

Cryopreservation of climbing perch (Anabas testudineus) sperm has been developed by several researchers. However, the post-cryopreservation sperm quality obtained remains suboptimal and can potentially be improved through optimization of the pre-freezing and thawing techniques. Therefore, this study aimed to determine the best pre-freezing and thawing techniques for climbing perch sperm cryopreservation. This research was conducted from March to April 2025 at the Fish Hatchery and Breeding Laboratory, Faculty of Marine and Fisheries, Syiah Kuala University. The study applied a non-factorial Completely Randomized Design (CRD) and was carried out in three stages. The first stage evaluated the washing/immersion of climbing perch eggs in EM-4 solution using five treatments: EM-4 dosage of 0 ml/L (control), 0.5 ml/L, 1.0 ml/L, 1.5 ml/L, and 2.0 ml/L. The second stage tested several pre-freezing techniques with different temperature gradients, namely: F1 (4°C, 0°C, −79°C, and −196°C), F2 (4°C, 0°C, and −196°C), F3 (4°C, −79°C, and −196°C), and F4 (4°C and −196°C). The third stage evaluated thawing techniques with different thawing temperatures and durations, namely 25°C, 30°C, and 35°C, each with immersion times of 5, 10, and 15 minutes. The results of the first experiment showed that egg immersion in EM-4 at a dosage of 1.0 ml/L was the best treatment. The results of the second experiment indicated that the pre-freezing temperature significantly affected sperm motility, viability, abnormality, and egg fertilization rate (P0.05). The best post-cryopreservation sperm quality was obtained in treatment F1, i.e., the pre-freezing technique with a temperature gradient of 4°C, 0°C, −79°C, and −196°C, resulting in sperm motility of 78.50 ± 1.70%, viability of 77.50 ± 1.91%, abnormality of 1.50 ± 0.57%, fertilization rate of 62.50 ± 8.18%, and sperm lifespan of 9.82 ± 1.58 minutes. In the third experiment, the thawing technique significantly affected post-cryopreservation sperm motility, viability, abnormality, fertilization rate, and sperm lifespan (P