Electronic Theses and Dissertation

Infeksi jamur Candida albicans masih menjadi permasalahan kesehatan pada manusia dan hewan, terutama di wilayah tropis seperti Indonesia. Resistensi terhadap obat antijamur sintetis semakin meningkat sehingga diperlukan alternatif pengobatan berbahan alami. Salah satu bahan herbal yang berpotensi adalah tumbuhan sarang semut (Hydnophytum formicarum). Penelitian ini bertujuan mengetahui efektivitas ekstrak metanol umbi Hydnophytum formicarum asal Aceh Besar dalam menghambat pertumbuhan Candida albicans ATCC 10231. Penelitian dilakukan secara eksperimental menggunakan metode difusi cakram dengan empat konsentrasi ekstrak, yaitu 6,25%, 12,5%, 25%, dan 50%, serta kontrol positif (itrakonazol 8 µg) dan kontrol negatif (CMC 1%). Setiap perlakuan dilakukan tiga ulangan. Parameter yang diamati berupa diameter zona hambat pada media Saboraud Dextrose Agar (SDA) setelah inkubasi 24 jam pada suhu 37°C. Data dianalisis menggunakan ANOVA dilanjutkan uji Duncan pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa ekstrak metanol tumbuhan Hydnophytum formicarum dapat menghambat pertumbuhan Candida albicans ATCC 10231, namun masih tergolong memiliki daya hambat lemah. Rata-rata zona hambat berturut-turut adalah 6,43±0,90 mm (6,25%), 6,67±0,50 mm (12,5%), 7,28±0,77 mm (25%), dan 8,70±1,41 mm (50%). Kontrol positif menghasilkan zona hambat kuat, yaitu 28,96±1,53 mm, sedangkan kontrol negatif tidak menunjukkan aktivitas hambat. Terdapat perbedaan yang signifikan antarperlakuan (p

Candida albicans infection remains a significant health concern in both humans and animals, particularly in tropical regions such as Indonesia. Increasing resistance to conventional antifungal drugs indicates the need for alternative natural treatments. One of which is the ant nest plant (Hydnophytum formicarum). This study aimed to assess the antifungal effectiveness of methanol extract from the tubers of H. formicarum originating from Aceh Besar in inhibiting the growth of Candida albicans ATCC 10231. The study was conducted using the disk diffusion method with four extract concentrations: 6.25%, 12.5%, 25%, and 50%. 8 µg of itraconazole served as the positive control, while 1% CMC was used the negative control. Each treatment was performed in triplicate. The parameter observed was the diameter of the inhibition zone on SDA medium after 24 hours of incubation at 37°C. Data were analyzed using ANOVA followed by Duncan’s multiple range test at a 95% confidence interval. The results showed that the methanol extract of H. formicarum was able to inhibit the growth of C. albicans, although the inhibitory activity was categorized as weak. The mean inhibition zones obtained were 6.43±0.90 mm (6.25%), 6.67±0.50 mm (12.5%), 7.28±0.77 mm (25%), and 8.70±1.41 mm (50%). The positive control produced a strong inhibition zone of 28.96±1.53 mm, while the negative control showed no inhibitory activity. Statistical analysis indicated significant differences among treatments (p