Electronic Theses and Dissertation

HISTOMORFOMETRI TIMUS AYAM BROILER YANG DIBERI PAKAN TEPUNG MAGGOT BLACK SOLDIER FLY (BSF)
Intan Rifacallista (2202101010063)
Prof. Dr. drh. Sri Wahyuni, M.Si.
drh. Lailia Dwi Kusuma Wardani, M.Si
ABSTRAK
Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tepung maggot Black Soldier Fly terhadap histomorfometri timus ayam broiler umur berbeda. Penelitian ini menggunakan rancangan acak lengkap dengan 2 faktor yaitu perlakuan maggot BSF dan umur ayam. Sampel penelitian menggunakan Day Old Chick (DOC). Faktor perlakuan maggot BSF sebanyak 4 kelompok terdiri dari P0 (100 % pakan komersil), P1 (90% Pakan komersil + 10% tepung maggot BSF), P2 (80 % pakan komersil + 20% tepung Maggot BSF), dan P3 (70% pakan komersil + 30% tepung maggot BSF). Kelompok faktor umur ayam berupa umur 14 hari dan 21 hari. Perlakuan penelitian selama 14 hari. Sampel ayam dinekropsi untuk diambil organ timus. Sampel organ dibuat preparat histologi dan dengan pewarnaan Hematoksilin Eosin. Data hasil pengukuran lebar korteks dan medula lobular timus dianalisis menggunakan uji Two-Way ANOVA dan uji lanjutan Tukey test. Hasil terlebar korteks umur 14 hari pada P3 (373,59±13,47μm) dan umur 21 hari pada P2 (346,9±35,87μm). Lebar medula terbesar umur 14 hari pada P3 (538,82±44,32μm) dan umur 21 hari pada P2 (425,52±16,63μm). Rerata lebar korteks pada umur 14 hari sejumlah (313,5±43,24) sedangkan rerata lebar korteks pada 21 hari sejumlah (294,67±53,98μm). Rerata lebar medula lobular pada umur 14 hari sejumlah (404,99±102,36μm) dan hasil rerata lebar medula lobular pada umur 21 hari sejumlah (359,50±51,66μm). Baik faktor umur dan perlakuan maggot memiliki interaksi antar keduanya. Kesimpulan dari studi ini menunjukan bahwa pemberian pakan tepung maggot BSF memberikan pengaruh positif terhadap histomorfometri timus dengan perlakuan terbaik pada P3 pada umur 14 hari dan P2 pada umur 21 hari.
Kata kunci: Ayam broiler, histomorfometri, maggot BSF, timus.
 

THYMUS HISTOMORPHOMETRY OF BROILERS SUPPLEMENTED WITH BLACK SOLDIER FLY (BSF) MAGGOT MEAL ABSTRACT This study aims to determine the effect of Black Soldier Fly maggot on histomorphometry of thymus of broiler with different ages. This study used a completely randomized design with 2 factors, namely BSF maggot treatment and chicken age. The research sample used Day Old Chick (DOC). The BSF maggot treatment factor was 4 groups consisting of P0 (100% commercial feed), P1 (90% Commercial feed + 10% BSF maggot flour), P2 (80% commercial feed + 20% BSF Maggot flour), and P3 (70% commercial feed + 30% BSF maggot flour). The chicken age factor group was 14 days and 21 days old. The research treatment lasted for 14 days. Organ samples were made histological preparations and stained with Haematoxilin Eosin. Data from measurements of the width of cortex and medulla of thymus lobular were analyzed using Two-Way ANOVA test and Tukey test. The result showed that The widest cortex at 14 days of age at P3 (373.59±13.47μm) and at 21 days of age at P2 (346.9±35.87μm). The largest medulla width at 14 days of age at P3 (538.82±44.32μm) and at 21 days of age at P2 (425.52±16.63μm). The average width of the cortex at 14 days of age was (313.5±43.24μm) while the average width of the cortex at 21 days was (294.67±53.98μm). The average width of the lobular medulla at 14 days of age was (404.99±102.36μm) and the average results of the lobular medulla width at 21 days of age were (359.50±51.66μm). Both age and maggot treatment interacted. The study concluded that BSF maggot positively affected thymus histomorphometry, with the best treatment being P3 at 14 days old and P2 at 21 days old. Keywords: Broiler chicken, histomorphometry, maggot BSF, thymus.