Electronic Theses and Dissertation

Pemanfaatan bahan alami dalam formulasi obat kumur terus dikembangkan sebagai alternatif yang lebih aman dan minim efek samping, salah satunya β-karoten dari crude palm oil (CPO) kelapa sawit (Elaeis guineensis Jacq.) yang memiliki aktivitas antioksidan dan antibakteri. Penelitian ini bertujuan untuk menguji karakteristik formula alami obat kumur berbasis β-karoten CPO serta mengevaluasi efektivitasnya terhadap pertumbuhan dan pembentukan biofilm Streptococcus mutans. Metode penelitian meliputi ekstraksi β-karoten dari CPO, formulasi obat kumur pada beberapa konsentrasi, uji karakteristik fisikokimia (pH dan sudut kontak), uji organoleptik, serta uji efektivitas terhadap laju pertumbuhan dan pembentukan biofilm Streptococcus mutans menggunakan metode microplate dengan pewarnaan kristal violet dan pengukuran absorbansi. Hasil penelitian menunjukkan bahwa formula obat kumur berbasis β-karoten CPO memenuhi kriteria sediaan obat kumur, memiliki stabilitas dan penerimaan organoleptik yang baik, serta mampu menurunkan laju pertumbuhan dan pembentukan biofilm Streptococcus mutans secara signifikan dibandingkan kontrol, dengan peningkatan konsentrasi β-karoten yang sejalan dengan peningkatan aktivitas antibakteri dan antibiofilm. Berdasarkan hasil tersebut, dapat disimpulkan bahwa obat kumur berbasis β-karoten CPO kelapa sawit (Elaeis guineensis Jacq.) berpotensi sebagai sediaan alami yang aman dan efektif dalam menghambat pertumbuhan serta pembentukan biofilm Streptococcus mutans.

The use of natural ingredients in mouthwash formulations has been increasingly explored as a safer alternative with minimal side effects, including β-carotene derived from crude palm oil (CPO) of oil palm (Elaeis guineensis Jacq.), which exhibits antioxidant and antibacterial properties. This study aimed to characterize a natural mouthwash formula based on β-carotene from CPO and to evaluate its effectiveness against the growth and biofilm formation of Streptococcus mutans. The study involved the extraction of β-carotene from CPO, formulation of the mouthwash at several concentrations, physicochemical characterization tests (pH and contact angle), organoleptic evaluation, and effectiveness testing on the growth rate and biofilm formation of Streptococcus mutans using a microplate method with crystal violet staining and absorbance measurement. The results demonstrated that the β-carotene–based mouthwash formula fulfilled the criteria for a mouthwash preparation, showed good stability and organoleptic acceptability, and significantly reduced the growth rate and biofilm formation of Streptococcus mutans compared to the control, with higher β-carotene concentrations associated with increased antibacterial and antibiofilm activities. These findings indicate that a mouthwash based on β-carotene from CPO of oil palm (Elaeis guineensis Jacq.) has the potential to be developed as a safe and effective natural preparation for inhibiting the growth and biofilm formation of Streptococcus mutans.