Electronic Theses and Dissertation

Candida albicans merupakan jamur yang secara normal hidup sebagai flora komensal di rongga mulut, namun dapat berkembang berlebihan dan menyebabkan kandidiasis oral ketika keseimbangan mikrobiota terganggu. Penatalaksanaan kandidiasis oral umumnya menggunakan obat kumur antimikroba sebagai terapi adjuvan, salah satunya adalah chlorhexidine gluconate (CHX). Meskipun demikian, penggunaan CHX dalam jangka panjang dapat menimbulkan efek samping seperti pewarnaan gigi (staining), sehingga diperlukan alternatif antijamur berbahan alami. Ekstrak etanol daun kari (Murraya koenigii) mengandung alkaloid, flavonoid, dan terpenoid yang berpotensi sebagai alternatif agen antijamur. Penelitian ini merupakan penelitian eksperimental laboratoris dengan desain post-test only control group yang bertujuan untuk menilai viabilitas C. albicans ATCC 10231 setelah paparan ekstrak etanol daun kari konsentrasi 3,125%, 6,25%, dan 12,5%, serta kontrol positif CHX 0,2%, pada waktu paparan 30 detik, 1 menit, dan 2 menit. Viabilitas dinilai berdasarkan nilai optical density (OD) pada panjang gelombang 600 nm menggunakan ELISA reader. Hasil analisis statistik uji Two-Way ANOVA menunjukkan bahwa konsentrasi dan waktu paparan berpengaruh signifikan terhadap viabilitas C. albicans (p=0,001), sedangkan interaksi antara konsentrasi dan waktu paparan tidak signifikan (p=0,473). Hasil uji lanjutan Tukey HSD menunjukkan perbedaan bermakna antar kelompok konsentrasi dan antar kelompok waktu paparan (p=0,001). Disimpulkan bahwa ekstrak etanol daun kari efektif menurunkan viabilitas C. albicans dengan efektivitas yang meningkat seiring kenaikan konsentrasi dan waktu paparan.

Candida albicans is a fungus that normally exists as a commensal flora in the oral cavity but can proliferate excessively and cause oral candidiasis when the oral microbiota balance is disrupted. Management of oral candidiasis commonly involves antimicrobial mouthwashes as adjuvant therapy, including chlorhexidine gluconate (CHX). However, long-term use of CHX may cause adverse effects such as tooth staining, highlighting the need for alternative natural antifungal agents. Ethanolic curry leaf (Murraya koenigii) extract contains alkaloids, flavonoids, and terpenoids with potential antifungal activity. This laboratory experimental study employed a post-test only control group design to evaluate the viability of C. albicans ATCC 10231 after exposure to ethanolic curry leaf extract at concentrations of 3.125%, 6.25%, and 12.5%, with 0.2% CHX as a positive control. Exposure times were 30 seconds, 1 minute, and 2 minutes. Viability was assessed based on optical density (OD) values measured at 600 nm using an ELISA reader. Two-Way ANOVA demonstrated that both concentration and exposure time significantly affected the viability of C. albicans (p=0.001), whereas their interaction was not significant (p=0.473). Tukey HSD post hoc test revealed significant differences among all concentration groups and among all exposure-time groups (p=0.001). It was concluded that ethanolic curry leaf extract effectively reduced the viability of C. albicans, with greater reductions observed at higher concentrations and longer exposure times.