Electronic Theses and Dissertation

Inflammatory Bowel Disease (IBD) merupakan penyakit inflamasi kronis saluran cerna yang bersifat kronik, kambuh dan remisi, dipengaruhi oleh interaksi kompleks antara faktor genetik, lingkungan, dan mikrobiota usus. Patogenesis IBD melibatkan interaksi kompleks antara mikrobiota komensal, sel epitel usus dan sel imun. Pasien IBD umumnya menunjukkan penurunan kadar asam lemak rantai pendek utama disertai dengan peningkatan kadar IL-6 serum. Penelitian ini bertujuan menganalisis hubungan antara kadar IL-6 serum dengan kadar asam lemak rantai pendek utama dan komponen utamanya, yaitu asam asetat, asam propionat, dan asam butirat, pada pasien IBD. Metode penelitian ini menggunakan desain potong lintang (cross-sectional) yang dilakukan pada 40 sampel pasien yang baru di diagnosis IBD. Kadar IL-6 serum diukur menggunakan metode Electro-chemiluminescence Immunoassay (ECLIA), dan kadar asam lemak rantai pendek utama dari sampel feses diukur menggunakan kromatografi gas. Analisis statistik antara kadar asam lemak rantai pendek utama dengan IL-6 serum dianalisis dengan uji regresi logistik, dan hubungan IL-6 serum dengan komponnen asam lemak rantai pendek utama, asam asetat, asam propionat, dan asam butirat menggunakan uji korelasi Spearman. Hasil penelitian menunjukkan bahwa pasien dengan kadar asam lemak rantai pendek utama rendah memiliki kemungkinan 6 kali lebih besar untuk mengalami peningkatan kadar IL-6 serum (OR = 6,066; p = 0,030). Kadar IL-6 serum dengan kadar asam butirat memperlihatkan korelasi negatif dengan kekuatan sedang yang bermakna secara statistik (R = -0,421; p = 0,007). Ditemukan pula korelasi negatif sangat lemah yang tidak bermakna antara IL-6 dengan asam propionat (R = -0,150; p = 0,355). Ditemukan korelasi positif lemah yang signifikan antara IL-6 dengan kadar asam asetat (R = 0,319; p = 0,045). Kesimpulan, kadar asam lemak rantai pendek utama yang rendah secara signifikan berhubungan dengan peningkatan kadar IL-6 serum. Hal ini menegaskan peran penting kadar asam lemak rantai pendek utama dalam patogenesis dan proses inflamasi pada IBD.
Kata Kunci: Inflammatory Bowel Disease, Interleukin 6, Asam Lemak Rantai Pendek

ASSOCIATION BETWEEN SERUM INTERLEUKIN 6 WITH MAJOR COMPONENTS OF SHORT-CHAIN FATTY ACIDS IN INFLAMMATORY BOWEL DISEASE PATIENTS Inflammatory Bowel Disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract characterized by periods of relapse and remission, influenced by complex interactions among genetic, environmental, and gut microbiota factors. The pathogenesis of IBD involves interactions between commensal microbiota, intestinal epithelial cells, and immune cells. Patients with IBD commonly exhibit decreased short-chain fatty acid (SCFA) levels accompanied by elevated serum IL-6 levels. This study aimed to analyze the association between serum IL-6 levels and total SCFA levels, as well as their main components—acetate, propionate, and butyrate—in patients with IBD. This cross-sectional study was conducted on 40 newly diagnosed IBD patients. Serum IL-6 levels were measured using the Electro-Chemiluminescence Immunoassay (ECLIA) method, while total SCFA levels from fecal samples were analyzed using gas chromatography. The association between total SCFA levels and serum IL-6 was evaluated using logistic regression analysis, and correlations between serum IL-6 and individual SCFAs (acetate, propionate, and butyrate) were assessed using Spearman’s correlation test. Patients with low total SCFA levels had a sixfold higher likelihood of elevated serum IL-6 levels (OR = 6.066; p = 0.030). Serum IL-6 showed a moderate, statistically significant negative correlation with butyrate levels (R = -0.421; p = 0.007). A very weak, non-significant negative correlation was found between IL-6 and propionate (R = -0.150; p = 0.355), while a weak but significant positive correlation was observed between IL-6 and acetate levels (R = 0.319; p = 0.045). Low total short-chain fatty acid levels are significantly associated with increased serum IL-6 levels, emphasizing the important role of SCFAs in the pathogenesis and inflammatory processes of IBD. Keywords: Inflammatory Bowel Disease, Interleukin-6, Short Chain Fatty Acids