Electronic Theses and Dissertation

Vitrifikasi merupakan suatu upaya untuk mempertahankan kualitas ovarium, akan tetapi selama vitrifikasi ovarium dapat mengalami kerusakan akibat syok osmotik. Follicle stimulating hormone (FSH) merupakan hormon yang memiliki peran penting dalam pertumbuhan dan perkembangan folikel, terutama sebagai antiapoptosis sel granulosa folikel, sementara luteinizing hormone (LH) adalah hormon gonadotropin tambahan yang bertanggung jawab atas pemeliharaan folikel selama vitrifikasi. FSH dan LH diproduksi oleh hipofisa bagian anterior. Penambahan ekstrak hipofisa ke dalam media vitrifikasi diharapkan dapat mempertahankan kualitas ovarium selama vitrifikasi.
Penelitian ini bertujuan untuk memperoleh informasi ilmiah tentang pengaruh penambahan ekstrak hipofisa kambing jantan lokal pada media vitrifikasi ovarium sapi aceh. Penelitian ini dilaksanakan di Laboratorium Pemuliaan dan Reproduksi Ternak, Fakultas Pertanian, Laboratorium Reproduksi dan Laboratorium Anatomi, Fakultas Kedokteran Hewan, dan Laboratotium Struktur dan Pengembangan Hewan B, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Syiah Kuala, Darussalam, Banda Aceh. Penelitian ini dilaksanakan pada bulan Juni - Agustus 2025. Penelitian ini menggunakan Rancangan Acak Lengkap Faktrorial (RALF) dengan kombinasi perlakuan konsentrasi EHK (ekstrak kelenjar hipofisa kambing) (0; 300; 600 μg/ml; BPE (Bovine pituitary extrack) 300 μg/ml), lama penyimpanan (0 hari dan 7 hari) dan 3 ulangan sehingga terdapat 24 unit percobaan. Parameter yang diamati pada penelitian ini adalah ekspresi FSHR dan LHR. Hasil penelitian dianalisis menggunakan uji Anova dan dilanjutkan dengan uji Duncan.
Hasil penelitian menunjukan bahwa morfometri ovarium kanan sapi aceh memiliki bobot 3,88 g, Panjang 2.26 cm dan lebar 1.58 cm. Ovarium sebelah kiri memiliki bobot 3,80 g, Panjang 2.27 cm dan lebar 1.68 cm. Hasil analisis menunjukkan bahwa penambahan ekstrak hipofisa kambing dan lama penyimpanan vitrifikasi berpengaruh nyata (P

Vitrification is an attempt to maintain ovarian quality, but during vitrification, the ovaries can be damaged due to osmotic shock. Follicle-stimulating hormone (FSH) is a hormone that plays an important role in follicle growth and development, especially as an anti-apoptosis of follicular granulosa cells, while luteinizing hormone (LH) is an additional gonadotropin hormone responsible for maintaining follicles during vitrification. FSH and LH are produced by the anterior pituitary gland. The addition of pituitary extract to the vitrification medium is expected to maintain ovarian quality during vitrification. This study aims to obtain scientific information about the effect of adding local male goat pituitary extract to the vitrification medium of Aceh cow ovaries. This study was conducted at the Animal Breeding and Reproduction Laboratory, Faculty of Agriculture, the Reproduction Laboratory and Anatomy Laboratory, Faculty of Veterinary Medicine, and the Animal Structure and Development Laboratory B, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Darussalam, Banda Aceh. This study was conducted from June to August 2025. This study used a Complete Randomized Factorial Design (CRFD) with a combination of treatments of EHK (goat pituitary gland extract) concentration (0; 300; 600 μg/ml; BPE (Bovine pituitary extract) 300 μg/ml), storage duration (0 days and 7 days), and 3 replications, resulting in 24 experimental units. The parameters observed in this study were FSHR and LHR expression. The results were analyzed using ANOVA and followed by Duncan's test. The results showed that the right ovary of Aceh cattle had a weight of 3.88 g, a length of 2.26 cm, and a width of 1.58 cm. The left ovary had a weight of 3.80 g, a length of 2.27 cm, and a width of 1.68 cm. The analysis results showed that the addition of goat pituitary extract and the duration of vitrification storage had a significant effect (P