Electronic Theses and Dissertation

Penelitian ini bertujuan untuk menguji aktivitas antioksidan ekstrak metanol, n-heksana, etil asetat, dan metanol partisi bunga Cassia siamea Lamk. serta memformulasikan ekstrak aktif menjadi sediaan lotion yang stabil dan berpotensi sebagai antioksidan. Cassia siamea, anggota famili Fabaceae, dikenal luas dalam pengobatan tradisional dan diyakini mengandung berbagai senyawa bioaktif. Berat ekstrak metanol, n-heksana, etil asetat, dan metanol partisi bunga Cassia siamea diperoleh dari proses maserasi adalah 371,789; 210,491; 28,076; dan 109,247 (g). Pengujian aktivitas antioksidan dilakukan dengan menggunakan metode 1 diphenyl-2-pychrilhidrazil (DPPH). Nilai inhibitory concentration (IC50) yang diperoleh adalah sebesar 66,76; 100,9; 239,95; dan 75,97 (ppm) untuk ekstrak metanol, etil asetat, n-heksana dan metanol partisi secara berturut-turut. Lotion dibuat dengan variasi konsentrasi ekstrak metanol bunga C. siamea lalu diuji evaluasi fisik, aktivitas antioksidan dan nilai Sun Protection Factor (SPF) dari masing-masing formula. Hasil evaluasi fisik untuk semua formula menunjukkan telah memenuhi standar SNI 2016-4399-1996. Hasil uji aktivitas antioksidan pada sediaan lotion menunjukkan nilai IC50 yang lebih tinggi, yaitu F1 441,37 ppm, F2 234,42 ppm dan F3 189 ppm. Hasil uji SPF pada masing-masing formula menunjukkan F3 memiliki nilai SPF tertinggi sebesar 31,62. Lotion F2 dan F1 memiliki nilai SPF 28,82 dan 25,82 yang juga termasuk kategori proteksi tinggi.

Kata kunci: Cassia siamea L, Antioksidan, Lotion, 1,1 diphenyl-2-pychrilhidrazil (DPPH), inhibitory concentration (IC50), SPF.

ABSTRACT This research aimed to examine the antioxidant activity of methanol, n-hexane, ethyl acetate, and partitioned methanol extracts of Cassia siamea Lamk. flowers and to formulate the active extract into a stable lotion with antioxidant potential. Cassia siamea, a member of the Fabaceae family, is widely known in traditional medicine and is believed to contain various bioactive compounds. The research stages included methanol extraction of C. siamea flowers using the maceration process. The weights of the methanol, n-hexane, ethyl acetate, and partitioned methanol extracts obtained were 371.789 g, 210.491 g, 28.076 g, and 109.247 g, respectively. Antioxidant activity testing was carried out using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The inhibitory concentration (IC50) values obtained were 66.76 ppm, 100.9 ppm, 239.95 ppm, and 75.97 ppm for the methanol, ethyl acetate, n-hexane, and partitioned methanol extracts, respectively. Lotions were prepared with variations in the concentration of C. siamea flower methanol extract and then subjected to physical evaluation, antioxidant activity testing, and Sun Protection Factor (SPF) value assessment. The results of the physical evaluation for all formulas showed that they met the SNI 2016-4399-1996 standard. The results of the antioxidant activity test on the lotion preparations showed higher IC50 values, namely F1 at 441.37 ppm, F2 at 234.42 ppm, and F3 at 189 ppm. The SPF test results for each formula showed that F3 had the highest SPF value of 31.62. Lotions F2 and F1 had SPF values of 28.82 and 25.82, respectively. Keywords: Cassia siamea L, Antioxidant, Lotion, 1,1-diphenyl-2-picrylhydrazyl (DPPH), Inhibitory Concentration (IC50), SPF.