Electronic Theses and Dissertation

Kriopreservasi merupakan salah satu teknologi penting dalam bidang reproduksi ikan yang berperan dalam mendukung ketersediaan benih unggul secara berkelanjutan serta pelestarian sumber daya genetik perikanan. Ikan lele lokal Clarias batrachus merupakan salah satu spesies asli yang memiliki nilai ekonomi tinggi dan tersebar luas di Indonesia. Namun, budidaya spesies ini masih menghadapi berbagai kendala, termasuk keterbatasan teknologi domestikasi dan reproduksi. Di sisi lain, rainbow trout Oncorhynchus mykiss sebagai spesies introduksi dari Amerika Utara dan Kanada, telah berhasil dibudidayakan di beberapa negara Asia dan Eropa, termasuk Indonesia, meskipun masih memerlukan optimalisasi pada aspek reproduktifnya. Penelitian ini bertujuan untuk mengeksplorasi berbagai jenis dan konsentrasi krioprotektan serta antioksidan pada kriopreservasi sperma ikan lele lokal dan ikan rainbow trout. Penelitian dilakukan dalam empat tahap dengan menggunakan Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan bahwa jenis dan konsentrasi krioprotektan maupun antioksidan memberikan pengaruh yang signifikan (P

Cryopreservation is one of the important technologies in fish reproduction that plays a role in supporting the sustainable availability of high-quality broodstock and the conservation of fish genetic resources. This technology allows long-term storage of genetic material, particularly sperm, while maintaining its viability and fertility. Local catfish Clarias batrachus is one of the native species that has high economic value and wide distribution across Indonesia. However, the aquaculture of this species still faces various challenges, including limitations in domestication and reproduction technology. On the other hand, rainbow trout Oncorhynchus mykiss as an introduced species from North America and Canada, has been successfully cultured in several Asian and European countries, including Indonesia, although its reproductive aspects still require further optimization. This study aims to evaluate the effectiveness of various types and concentrations of cryoprotectants and antioxidants in the cryopreservation of local catfish and rainbow trout sperm. The research was conducted in four stages using a Completely Randomized Design. The results showed that the type and concentration of cryoprotectants and antioxidants had a significant effect (P