Electronic Theses and Dissertation

Penelitian ini dilakukan untuk mengevaluasi aktivitas anti-aging serta merumuskan sediaan krim dari ekstrak bunga Johar (Cassia siamea L.) yang diperoleh menggunakan pelarut metanol, etil asetat, dan n-heksan. Proses ekstraksi dilakukan melalui metode maserasi dengan pelarut metanol, kemudian diikuti dengan partisi cair-cair untuk memperoleh ekstrak berdasarkan tingkat kepolarannya. Kandungan senyawa metabolit sekunder seperti alkaloid, saponin, flavonoid, fenolik, steroid, terpenoid, dan tanin berhasil diidentifikasi melalui uji fitokimia. Aktivitas anti-aging dievaluasi secara in-vitro dengan mengukur penghambatan enzim elastase pankreas babi (Porcine Pancreatic Elastase/PPE), dan asam oleanolat digunakan sebagai kontrol positif. Dari hasil pengujian, ditemukan bahwa ekstrak metanol kasar menunjukkan aktivitas penghambatan elastase tertinggi dengan persen inhibisi sebesar 97,53% dan nilai IC₅₀ sebesar 13,895 µg/mL. Aktivitas ini diikuti oleh ekstrak metanol partisi (IC₅₀ 22,292 µg/mL), etil asetat (IC₅₀ 24,931 µg/mL), dan n-heksan (IC₅₀ 38,597 µg/mL). Sediaan krim yang diformulasikan dari ekstrak metanol kasar menunjukkan karakteristik fisik yang sesuai dengan standar SNI-2016-4399-1996, mencakup nilai pH, daya sebar, daya lekat, homogenitas, dan viskositas. Formula terbaik ditunjukkan oleh formula 3 (F3), yang diformulasikan dengan konsentrasi ekstrak tertinggi dan menghasilkan nilai IC₅₀ sebesar 95,488 µg/mL. Dengan demikian, dapat disimpulkan bahwa ekstrak metanol kasar dari bunga Johar berpotensi digunakan sebagai agen anti-aging alami yang efektif dalam sediaan krim topikal.

Kata kunci: Cassia siamea L., anti-aging, Porcine Pancreatic Elastase/PPE, IC50, formulasi.

This research was conducted to evaluate the anti-aging activity and formulate a cream preparation from Johar flower (Cassia siamea L.) extracts using methanol, ethyl acetate, and n-hexane as solvents. Extraction was carried out by maceration using methanol, followed by liquid-liquid partitioning to obtain extracts with different polarities. The presence of secondary metabolites such as alkaloids, saponins, flavonoids, phenolics, steroids, terpenoids, and tannins was identified through phytochemical screening. Anti-aging activity was assessed in vitro by measuring the inhibition of porcine pancreatic elastase (PPE), with oleanolic acid used as a positive control. The results showed that the crude methanol extract exhibited the highest elastase inhibitory activity, with a percentage inhibition of 97.53% and an IC₅₀ value of 13.895 µg/mL. This was followed by the partitioned methanol extract (IC₅₀ 22.292 µg/mL), ethyl acetate extract (IC₅₀ 24.931 µg/mL), and n-hexane extract (IC₅₀ 38.597 µg/mL). The cream formulated from the crude methanol extract was found to meet the physical criteria set by the Indonesian National Standard (SNI-2016-4399-1996), including pH, spreadability, adhesion, homogeneity, and viscosity. The best anti-aging performance was shown by Formula 3 (F3), which contained the highest extract concentration and achieved an IC₅₀ value of 95.488 µg/mL. It can be concluded that the crude methanol extract of Johar flower has promising potential as a natural anti-aging agent in topical cream formulations. Keywords: Cassia siamea L., anti-aging, Porcine Pancreatic Elastase (PPE), IC50, formulation.