Electronic Theses and Dissertation

Aktinobakteri merupakan kelompok mikroorganisme yang diisolasi dari tanah, dikenal sebagai kelompok bakteri gram-positif, morfologi filamennya yang menyerupai jamur, dikenal mampu menghasilkan antibiotik, dan sebagai inhibitor tirosinase. Penelitian ini bertujuan untuk mengetahui aktivitas potensi dari aktinobakteri sebagai inhibitor tirosinase, menganalisis senyawa metabolit sekunder aktinobakteri secara molekuler. dan Mengidentifikasi gen 16S rRNA isolat aktinobakteri. Metode yang digunakan pada penelitian ini adalah metode deskriptif dan eksperimental. Nilai absorbansinya diukur menggunakan alat Elisa reader. Analisis senyawa bioaktif dari isolat terpilih dilakukan menggunakan Gas Chromatography–Mass Spectrometry (GC-MS), Identifikasi karakteristik isolat aktinobakteri tanah dilakukan berdasarkan gen 16S rRNA. Hasil yang diperoleh menunjukkan isolat ATS 14 mampu menghambat tirosinase pada nilai persentase inhibisi 52,74%. Analisis GC-MS mendeteksi sebanyak 13 senyawa dipilih hanya ada 1 senyawa yang sudah terbukti yaitu senyawa Oleic Acid yang berperan sebagai inhibitor tirosinase. Isolat ATS14 memiliki kemiripan sekuens sebesar 99,92% dengan Streptomyces sp. strain S15_SBA.

Kata kunci: Aktinobakteri, Inhibitor Tirosinase, Gas Chromatography–Mass Spectrometry (GC-MS), Gen 16S rRNA, Streptomyces sp.

Actinobacteria are a group of microorganisms isolated from soil, known as a diverse group of Gram-positive bacteria with filamentous morphology resembling fungi. They are recognized for their ability to produce antibiotics and act as tyrosinase inhibitors. This study aimed to investigate the potential activity of actinobacteria as tyrosinase inhibitors, to analyze their secondary metabolite compounds at the molecular level, and to identify the 16S rRNA gene of the actinobacterial isolates. The methods employed were descriptive and experimental. Absorbance values were measured using an ELISA reader. Bioactive compound analysis of the selected isolate was conducted using Gas Chromatography–Mass Spectrometry (GC-MS), Identification of the actinobacterial isolates was based on the 16S rRNA gene. The results showed that isolate ATS14 inhibited tyrosinase with a percentage inhibition of 52.74%. GC-MS analysis detected 13 compounds and only one were previously reported as tyrosinase inhibitors: Oleic Acid. The 16S rRNA gene analysis revealed that isolate ATS14 shared 99.92% sequence similarity with Streptomyces sp. strain S15_SBA. Keywords: Actinobacteria, Tyrosinase inhibitor, Gas Chromatography–Mass Spectrometry (GC-MS), 16S rRNA gene, Streptomyces sp