Electronic Theses and Dissertation

Latar Belakang: Pembentukan radikal bebas akibat paparan sinar UVB memicu aktivasi dan ekspresi matrix metalloproteinase-1 (MMP-1), yang berkontribus terhadap degradasi kolagen kulit dan proses photoaging. Holothuriidae (teripang hitam) diketahui mengandung senyawa bioaktif dengan aktivitas antioksidan dan fotoprotektif terhadap jaringan kulit, sehingga berpotensi sebagai agen anti photoaging. Tujuan: Penelitian ini bertujuan untuk mengevaluasi efek krim ekstrak etanol teripang hitam Holothuriidae terhadap densitas kolagen dan kadar MMP-1 pada tikus Wistar jantan yang dipapar sinar UVB. Metode: Studi eksperimental in vivo melibatkan 16 ekor tikus yang dibagi menjadi empat kelompok: kontrol negatif, kontrol positif (krim vitamin C 3%), serta dua kelompok perlakuan yang diberikan krim ekstrak etanol Holothuriidae dengan konsentrasi 2,5% dan 5%. Hasil: Krim ekstrak Holothuriidae menunjukkan efek penurunan kadar MMP-1 yang signifikan pada konsentrasi 5% dengan perbedaan rerata dibandingkan dengan kontrol negatif sebesar 59,5 ± 17,60pg/mL. Hasil juga menunjukkan peningkatan densitas kolagen yang signifikan pada konsentrasi 5%, dengan perbedaan rerata densitas kolagen 17,52 ± 1,57% dibandingkan dengan kontrol negatif. Kesimpulan: Krim ekstrak etanol teripang hitam pada kadar 5% berpotensi sebagai agen anti-photoaging melalui mekanisme penghambatan MMP-1 dan stimulasi sintesis kolagen pada kulit yang terpapar sinar UVB. Penelitian lanjutan diperlukan untuk mengevaluasi bioavailabilitas dan efektivitas pada manusia, serta memahami interaksi senyawa bioaktif dalam mikrolingkungan kulit.

Kata kunci : Photoaging, Holothuriidae, kolagen, MMP-1, in-vivo

Background: Free radicals formation due to UVB exposure triggers the activation and expression of matrix metalloproteinase-1 (MMP-1), contributes to collagen degradation and photoaging. Holothuriidae (black sea cucumber) is known to contain bioactive compounds with antioxidant and photoprotective activities on skin tissue, thus it has potential as an anti-photoaging agent. Objective: To evaluate the effect of Holothuriidae ethanol extract cream on collagen density and MMP-1 levels in male Wistar rats exposed to UVB light. Methods: An in vivo study was conducted using 16 rats assigned to four groups: a negative control group olny exposed to UVB radiation, a positive control group receiving topical administration of 3% Vitamin C, and two experimental groups treated with Holothuriidae extract cream at concentrations of 2.5% and 5%. Collagen density and MMP-1 expression levels were quantified using histological and ELISA. Results: Holothuriidae extract cream showed a significant effect on reducing MMP-1 concentration at a concentration of 5% with a mean difference compared to the negative control of 59.5 ± 17.60pg/mL. The results also showed a significant increase in collagen density at a concentration of 5%, with a mean difference in of 17.52 ± 1.57% compared to the negative control. Conclusion: Topical application of 5% ethanol extract of Holothuriidae demonstrates potential as an anti-photoaging agent, exerting inhibitory effects on MMP-1 expression and promoting collagen synthesis in UVB-irradiated rat models. Further research is needed to evaluate bioavailability and effectiveness in humans, as well as to understand the interactions of bioactive compounds in the skin microenvironment. Keywords : Photoaging, Holothuriidae, collagen, MMP-1, in-vivo