Electronic Theses and Dissertation

Latar Belakang: Penuaan kulit, yang ditandai dengan penurunan elastisitas, merupakan masalah dermatologis global. Penelitian ini mengeksplorasi potensi ekstrak biji alpukat (Persea americana Mill) sebagai agen antiaging alami. Kaya akan antioksidan, biji alpukat diduga mampu menghambat degradasi elastin sehingga memperlambat proses penuaan kulit. Aktivitas antioksidan dan anti elastase dari ekstrak ini dievaluasi melalui pendekatan in silico dan in vitro. Metode: Ekstrak etanol biji alpukat dari Bener Meriah, Aceh, diperoleh melalui metode maserasi menggunakan etanol 70%. Skrining fitokimia dilakukan untuk mengidentifikasi senyawa bioaktif. Aktivitas antioksidan diukur menggunakan uji DPPH, sementara aktivitas anti elastase diuji secara in silico melalui molecular docking terhadap enzim porcine pancreatic elastase (PPE) dan secara in vitro menggunakan substrat SANA. Hasil: Ekstrak etanol biji alpukat menunjukkan aktivitas antioksidan yang sangat kuat dengan nilai IC₅₀ sebesar 47,31 ppm. Selain itu, ekstrak ini juga menunjukkan aktivitas anti elastase yang signifikan dengan nilai IC₅₀ sebesar 6,980 ppm secara in vitro. Dalam analisis in silico, senyawa Cholestan-3-ol, 2-methylene-, (3β,5α)- menunjukkan afinitas ikatan sebesar -8,0 kcal/mol, yang sebanding dengan ligan pembanding. Analisis fisikokimia menunjukkan Cholestan-3-ol, 2-methylene-, (3β,5α)- memiliki 1 pelanggaran aturan lipinksi, tidak menembus sawar darah otak, tidak diabsorbsi oleh gastrointestinal dan permeabilitas kulit yang baik. Kesimpulan: Ekstrak etanol biji alpukat memiliki aktivitas antioksidan yang sangat kuat serta aktivitas anti elastase yang signifikan, sehingga berpotensi digunakan sebagai agen antiaging alami.

Kata Kunci: Antiaging, Persea americana Mill, porcine pancreatic elastase, in silico, in vitro

Background: Skin aging, marked by reduced elasticity, is a global aesthetic and health concern. This study investigates the potential of avocado (Persea americana Mill. var. Miki) seed ethanol extract as a natural anti-aging agent due to its high antioxidant content, which may inhibit elastin degradation. Methods: A combination of in silico and in vitro methods was employed to evaluate the antioxidant and anti-elastase activities of the extract. The seeds were collected from Bener Meriah, Aceh, and extracted using 70% ethanol via maceration. Phytochemical screening was conducted to identify bioactive compounds. Antioxidant activity was determined using the DPPH assay, while anti-elastase activity was assessed in silico through molecular docking with the porcine pancreatic elastase (PPE) enzyme, and in vitro using PPE and the SANA substrate. Results: The extract demonstrated very strong antioxidant activity with an IC₅₀ value of 47.31 ppm and effectively inhibited the PPE enzyme in vitro with an IC₅₀ of 6.980 ppm. Molecular docking analysis showed that Cholestan-3-ol, 2-methylene-, (3β,5α)-, a compound identified in the extract, had a binding affinity of −8.0 kcal/mol, comparable to the control. Physicochemical profiling revealed one Lipinski's rule violation. The compound does not penetrate the blood-brain barrier, is not absorbed gastrointestinally, but exhibits good skin permeability. Conclusion: Avocado seed ethanol extract demonstrates potent antioxidant and anti-elastase activities, supporting its potential use as a natural anti-aging agent. Keywords: Antiaging, Persea americana Mill, porcine pancreatic elastase, in silico, in vitro