Electronic Theses and Dissertation

Gingiva merupakan jaringan lunak di rongga mulut yang mudah mengalami luka akibat trauma fisik maupun prosedur medis. Penyembuhan luka melibatkan proses kompleks, salah satunya adalah sintesis kolagen oleh fibroblas. Bonggol nanas (Ananas comosus (L) Merr) diketahui mengandung flavonoid, tanin, alkaloid, fenolik, dan enzim bromelin yang berpotensi mempercepat penyembuhan luka. Penelitian ini bertujuan untuk menguji efektivitas gel ekstrak bonggol nanas terhadap jumlah kolagen pada luka gingiva. Desain penelitian menggunakan post-test only control group design dengan 9 ekor tikus Wistar jantan yang dibagi menjadi tiga kelompok: kelompok perlakuan (gel ekstrak bonggol nanas 15%), kontrol positif (aloclair gel), dan kontrol negatif (carbopol). Gel dioleskan dua kali sehari selama 14 hari. Preparat histologi diwarnai dengan Picrosirius Red dan jumlah kolagen dianalisis menggunakan mikroskop pembesaran 400x dan software ImageJ. Hasil menunjukkan bahwa kelompok perlakuan memiliki jumlah kolagen tertinggi (4,03), diikuti kontrol positif (3,5) dan kontrol negatif (3,12), namun analisis statistik ANOVA menunjukkan tidak terdapat perbedaan yang signifikan antar kelompok (p=0,235). Kesimpulan dari penelitian ini adalah gel ekstrak bonggol nanas 15% memiliki potensi meningkatkan jumlah kolagen pada luka gingiva, meskipun tidak signifikan secara statistik.

Kata kunci: Ekstrak bonggol nanas (Ananas comosus (L) Merr), kolagen, luka
gingiva

The gingiva is a soft tissue in the oral cavity that is susceptible to injury due to mechanical trauma or dental procedures. The wound healing process heavily relies on the activity of fibroblasts in producing collagen, which serves as a key component of connective tissue. Pineapple core (Ananas comosus (L) Merr) contains active compounds such as flavonoids, tannins, alkaloids, phenolics, and the enzyme bromelain, which are known to accelerate wound healing by enhancing collagen synthesis. This study aimed to determine the effectiveness of pineapple core extract gel on collagen quantity in gingival wounds. This true experimental study employed a post-test only control group design using nine male Wistar rats divided into three groups: the treatment group (15% pineapple core extract gel), positive control (aloclair gel), and negative control (carbopol). The gel was applied twice daily for 14 days. On day 14, the rats were euthanized, and gingival tissue was collected to prepare histological slides stained with Picrosirius Red. Collagen levels were observed under a light microscope at 400x magnification across five fields of view and analyzed using ImageJ software. The results showed that the treatment group had the highest mean collagen amount (4.03), followed by the positive control (3.5) and negative control (3.12), although ANOVA analysis revealed no statistically significant difference among the groups (p=0.235). It can be concluded that 15% pineapple core extract gel has the potential to increase collagen in gingival wounds, although the effect was not statistically significant. Keywords: Pineapple core (Ananas comosus (L) Merr), collagen, gingiva wound