Electronic Theses and Dissertation

Severe Early Childhood Caries (S-ECC) merupakan bentuk karies gigi yang menyerang anak usia dini dan berkaitan erat dengan bakteri Streptococcus mutans. Rosemary (Salvia rosmarinus) merupakan tanaman herbal yang diketahui memiliki senyawa aktif seperti flavonoid dan fenolik yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk menguji aktivitas antibakteri ekstrak daun rosemary terhadap Streptococcus mutans yang diisolasi dari plak anak S-ECC. Penelitian ini merupakan studi eksperimental laboratoris dengan desain post-test only control group. Ekstrak diperoleh menggunakan metode maserasi dengan pelarut etanol 96%. Sampel S. mutans diambil dari plak satu anak penderita S-ECC dan dikultur di media TYS20B. Uji antibakteri dilakukan dengan metode difusi sumuran menggunakan konsentrasi ekstrak 25%, 50%, 75%, dan 100%, serta kontrol positif (klorheksidin 0,2%) dan kontrol negatif (akuades). Hasil menunjukkan adanya zona hambat pada semua konsentrasi, dengan rata-rata zona hambat tertinggi pada konsentrasi 100%. Hasil menunjukkan ekstrak daun rosemary memiliki potensi sebagai agen antibakteri alami terhadap Streptococcus mutans.

Kata kunci : Salvia rosmarinus, Severe Early Childhood Caries, Streptococcus mutans, aktivitas antibakteri.

Severe Early Childhood Caries (S-ECC) is a serious dental condition affecting young children and is closely linked to the presence of Streptococcus mutans. Rosemary (Salvia rosmarinus) is a medicinal plant known to contain bioactive compounds such as flavonoids and phenolics, which have demonstrated potential antibacterial effects. This study aimed to assess the antibacterial activity of rosemary leaf extract against Streptococcus mutans isolated from the dental plaque of children diagnosed with S-ECC. An experimental laboratory design with a post-test only control group was employed. The rosemary extract was prepared through maceration using 96% ethanol as the solvent. Streptococcus mutans samples were isolated from the plaque of a child with S-ECC and cultured on TYS20B medium. The antibacterial effect of the extract was evaluated using the well diffusion method at concentrations of 25%, 50%, 75%, and 100%. Chlorhexidine 0.2% served as the positive control, while distilled water was used as the negative control. The results demonstrated inhibition zones at all tested concentrations, with the largest mean inhibition observed at 100% extract concentration. These findings suggest that rosemary leaf extract possesses significant antibacterial properties against Streptococcus mutans. Keywords : Salvia rosmarinus, Severe Early Childhood Caries, Streptococcus mutans, antibacterial activity