Electronic Theses and Dissertation

Latar Belakang: Penggunaan gigi tiruan resin akrilik dapat meningkatkan risiko pertumbuhan Candida albicans, yang berpotensi menyebabkan infeksi oral seperti denture stomatitis. Tujuan Penelitian ini adalah untuk mengevaluasi efektivitas obat kumur berbahan dasar minyak nilam (Pogostemon cablin Benth.) dalam menghambat pertumbuhan Candida albicans pada pengguna gigi tiruan. Metode penelitian yang digunakan berupa eksperimental laboratorium dengan desain pretest-posttest controlled group. Analisis dilakukan dengan teknik kultur mikrobiologi sebelum dan sesudah dipaparkan obat kumur berbasis minyak nilam. Data yang diperoleh dianalisis menggunakan uji statistik Wilcoxon. Hasil penelitian: menunjukkan adanya penurunan signifikan dalam pertumbuhan koloni Candida albicans setelah pemaparan obat kumur minyak nilam. Sebanyak 18 responden mengalami penurunan jumlah koloni dengan mean rank sebesar 9,50, sementara tidak ada peningkatan jumlah koloni (mean rank 0,00). Uji statistik menunjukkan nilai Z = -3,726 dengan p-value = 0,000 (p < 0,05), yang mengindikasikan perbedaan signifikan sebelum dan sesudah. Kesimpulan: Obat kumur berbahan dasar minyak nilam efektif menurunkan jumlah koloni Candida albicans pada pengguna gigi tiruan. Koloni jamur tertinggi ditemukan pada pemakai gigi tiruan dengan durasi lebih dari 3 bulan hingga kurang dari 12 bulan.

Kata kunci: Antifungal, Candida albicans, Ekstrak nilam, Gigi tiruan,Obat kumur

Background:The use of acrylic resin dentures can increase the risk of Candida albicans growth, potentially leading to oral infections such as denture stomatitis. The purpose of this study was to evaluate the effectiveness of a mouthwash formulated with patchouli Oil (Pogostemon cablin Benth.) in inhibiting the growth of Candida albicans in denture wearers. The research method used was a laboratory experimental design with a pretest-posttest controlled group design. Analysis was conducted using microbiological culture techniques before and after the use of the patchouli oil-based mouthwash. The data obtained were analyzed using the Wilcoxon statistical test. Results: The study showed a significant decrease in the growth of Candida albicans colonies after using the patchouli oil mouthwash. A total of 18 respondents experienced a reduction in colony count with a mean rank of 9.50, while there was no increase in colony count (mean rank 0.00). Statistical testing showed a Z-value of -3.726 with a p-value of 0.000 (p < 0.05), indicating a significant difference before and after the use of the mouthwash. Conclusion: Mouthwash formulated with patchouli leaf oil effectively reduces the number of Candida albicans colonies in denture wearers. The highest fungal colony counts were observed in users with denture-wearing duration between more than 3 months and less than 12 months. Keywords: Antifungal, Candida albicans, Patchouli extract, Denture, Mouthwash