Electronic Theses and Dissertation

Tanaman waru laut (Thespesia populnea), yang termasuk dalam Family Malvaceae, merupakan salah satu jenis tanaman yang terdapat dalam Usada Taru Purnama (UTP) dan dikenal memiliki khasiat sebagai obat tradisional. Tanaman ini banyak ditemukan di berbagai wilayah di Indonesia, seperti di Pantai Alue Naga, Aceh. Penelitian ini bertujuan untuk mengetahui efektivitas dari ekstrak metanol daun waru laut (Thespesia populnea) dan menentukan LC50 larvasida Aedes aegypti. Metode ekstraksi menggunakan metode maserasi dengan jenis pelarut metanol selama 3x24 jam dan dipekatkan menggunakan Rotary Evaporator. Pengujian fitokimia dilakukan menggunakan uji kualitatif dan hasil toksisitas terhadap uji larvasida Aedes aegypti menggunakan beberapa konsentrasi ekstrak metanol daun waru laut (Thespesia populnea) diantaranya: 1.000 ppm, 500 ppm, 250 ppm, 125 ppm, 50 ppm, dan 25 ppm setiap perlakuannya. Terdapat 5 jenis senyawa metabolit sekunder yang didapatkan pada ekstrak metanol daun waru laut (Thespesia populnea) antara lain alkaloid, steroid, triterpenoid, saponin dan tanin. Hasil uji menunjukkan bahwa ekstrak daun waru laut Thespesia populnea memiliki tingkat toksisitas dengan kategori toksik sedang yaitu LC50 yaitu 848,6868 ppm.

Kata kunci : Waru laut Thespesia populnea, metabolit sekunder, ekstrasi, larvasida Aedes aegypti, LC50.

The sea hibiscus (Thespesia populnea), which belongs to the Malvaceae family, is one of the plants found in Usada Taru Purnama (UTP) and is known for its medicinal properties as a traditional medicine. This plant is widely found in various regions of Indonesia, such as at Alue Naga Beach in Aceh. This study aims to determine the effectiveness of methanol extract from sea hibiscus leaves (Thespesia populnea) and to determine the LC50 of the larvicide against Aedes aegypti. The extraction method used was maceration with methanol as the solvent for 3x24 hours, followed by concentration using a Rotary Evaporator. Phytochemical testing was conducted using qualitative tests, and toxicity testing against Aedes aegypti larvae was performed using various concentrations of methanol extract from waru laut leaves (Thespesia populnea), including: 1.000 ppm, 500 ppm, 250 ppm, 125 ppm, 50 ppm, and 25 ppm for each treatment. Five types of secondary metabolites were identified in the methanol extract of Thespesia populnea leaves, including alkaloids, steroids, triterpenoids, saponins, and tannins. The test results indicated that the Thespesia populnea leaf extract exhibited moderate toxicity with an LC50 value of 848,6868 ppm. Keywords: Thespesia populnea, secondary metabolites, extraction, Aedes aegypti larvicide, LC50