UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN BIDURI (CALOTROPIS GIGANTEA L.) TERHADAP ESCHERICHIA COLI | ELECTRONIC THESES AND DISSERTATION

Electronic Theses and Dissertation

Universitas Syiah Kuala

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UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN BIDURI (CALOTROPIS GIGANTEA L.) TERHADAP ESCHERICHIA COLI


Pengarang

Wira Nico Sempaty - Personal Name;

Dosen Pembimbing



Nomor Pokok Mahasiswa

1307101030147

Fakultas & Prodi

Fakultas / / PDDIKTI :

Subject
Penerbit

Banda Aceh : Fakultas Kedokteran., 2013

Bahasa

Indonesia

No Classification

615.321

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Infectious diseases are a major problem in the health sector in Indonesia. One
cause is the bacteria Escherichia coli. Uncontrolled use of antibiotics lead to
increased bacterial resistance to antibiotics. Traditional crops needed to solve the
problem proficiency level. Biduri leaves believed to be used as an antibacterial.
The purpose of this study is to investigate the antibacterial activity of leaf extracts
biduri (Calotropis gigantea L.) against Escherichia coli. Biduri leaves extracted
using 70% methanol by maceration. This research used Completely Randomized
Design (CRD) was divided into six treatment groups, consisting of the positive
control (chloramphenicol 30 mcg), negative control Dimethyl sulfoxide (DMSO)
10% and biduri leaf methanol extract at a concentration of 25%, 50%, 75% and
100% repeated 4 times. Data were analyzed using Analysis of Variance (ANOVA)
followed by Least Significant Difference test (LSD). The results showed that the
methanol extract of the leaves biduri have antibacterial activity in concentration
of 25%, 50%, 75%, and 100% with an average inhibition zone 6.12 mm, 6.75 mm,
7.62 mm and 8.75 mm. The results of ANOVA and LSD (p

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