Electronic Theses and Dissertation

Radikal bebas berperan dalam berbagai masalah kesehatan, sehingga diperlukan antioksidan eksogen. Medicated oil merupakan formulasi berbasis campuran minyak atsiri yang berpotensi sebagai antioksidan eksogen. Penelitian ini bertujuan untuk mengetahui karakteristik fisikokimia minyak atsiri tunggal (bergamot, pala, cendana, nilam, dan biji bunga matahari), mengevaluasi sediaan medicated oil (F1; F2; F3; F4 dan F5), serta menentukan aktivitas antioksidan minyak atsiri tunggal maupun sediaan medicated oil menggunakan metode ABTS. Hasil menunjukkan variasi aktivitas antioksidan pada minyak atsiri tunggal: pala (IC₅₀ 11,06 ppm) dan nilam (IC₅₀ 47,40 ppm) sangat kuat, cendana (IC₅₀ 111,46 ppm) dan bergamot (IC₅₀ 129,02 ppm) sedang, serta biji bunga matahari (IC₅₀ 154,11 ppm) lemah. Seluruh sediaan formulasi medicated oil juga berpotensi sebagai antioksidan: F2 (IC₅₀ 59,09 ppm) menunjukkan aktivitas terkuat, diikuti F1 (IC₅₀ 78,36 ppm) yang juga tergolong kuat, F3 (IC₅₀ 131,44 ppm) sedang, serta F4 (IC₅₀ 166,70 ppm) dan F5 (IC₅₀ 175,07 ppm) lemah. Hasil evaluasi sediaan medicated oil menunjukkan karakteristik fisikokimia yang baik dan tidak iritatif, meskipun pH belum optimal dan beberapa minyak atsiri belum sepenuhnya memenuhi standar. Disimpulkan bahwa minyak atsiri bergamot, pala, cendana, nilam dan biji bunga matahari beserta seluruh sediaan medicated oil memiliki potensi antioksidan, namun karakterisasi dan evaluasi menunjukkan perlunya optimasi lebih lanjut pada sediaan demi kesesuaian standar mutu dan pengembangan produk yang aman.

Kata Kunci: medicated oil, antioksidan, minyak atsiri, ABTS.

Free radicals play a role in various health problems, thus exogenous antioxidants are needed. Medicated oil is a formulation based on essential oil mixtures that have potential as exogenous antioxidants. This study aims to determine the physicochemical characteristics of single essential oils (bergamot, nutmeg, sandalwood, patchouli, and sunflower seed oil), evaluate medicated oil preparations (F1; F2; F3; F4 and F5), and determine the antioxidant activity of both single essential oils and medicated oil preparations using the ABTS method. Results showed variations in the antioxidant activity of single essential oils: nutmeg (IC₅₀ 11.06 ppm) and patchouli (IC₅₀ 47.40 ppm) were very strong; sandalwood (IC₅₀ 111.46 ppm) and bergamot (IC₅₀ 129.02 ppm) were moderate; and sunflower seed (IC₅₀ 154.11 ppm) was weak. All medicated oil formulations also showed antioxidant potential: F2 (IC₅₀ 59.09 ppm) exhibited the strongest activity, followed by F1 (IC₅₀ 78.36 ppm) which was also strong; F3 (IC₅₀ 131.44 ppm) was moderate; and F4 (IC₅₀ 166.70 ppm) and F5 (IC₅₀ 175.07 ppm) were weak. Physicochemical evaluation of the medicated oil formulations showed good and non-irritating characteristics, although the pH was not yet optimal and some essential oils did not fully meet standards. It is concluded that bergamot, nutmeg, sandalwood, patchouli, and sunflower seed essential oils along with all medicated oil formulations possess antioxidant potential, but further characterization and evaluation indicate the need for further optimization of the formulations for quality standard compliance and safe product development. Keywords: medicated oil, antioxidant, essential oil, ABTS.