Electronic Theses and Dissertation

Staphylococcus merupakan salah satu bakteri Gram positif yang menjadi penyebab infeksi pada manusia. Penelitian ini bertujuan untuk mengetahui distribusi Staphylococcus sp. berdasarkan spesimen klinik, jenis kelamin, usia dan ruang rawat serta melihat tingkat sensitivitas terhadap berbagai jenis antibiotik periode Januari 2020 hingga Maret 2025 di Laboratorium Mikrobiologi Klinik RSUD dr. Zainoel Abidin. Penelitian ini menggunakan metode observasi laboratorium dengan mengumpulkan data hasil kultur Staphylococcus sp. dari berbagai spesimen klinik. Data diambil secara retrospektif dan prospektif. Bakteri diisolasi pada media Blood Agar dan identifikasi serta uji kepekaan antibiotik dengan menggunakan alat VITEK ® 2 Compact. Hasil penelitian diperoleh 29 spesies Staphylococcus dari total 5.166 isolat yang teridentifikasi. Distribusi spesies yang paling dominan adalah Staphylococcus aureus yaitu 28,69%, diikuti Staphylococcus haemolyticus dan Staphylococcus hominis ssp hominis masing-masing 23,87% dan 19,82%. Distribusi Staphylococcus sp. berdasarkan spesimen klinik paling banyak ditemukan pada spesimen darah yaitu 52,86%, diikuti spesimen pus dan spesimen cairan masing-masing 17,13% dan 12,27%. Distribusi Staphylococcus sp. berdasarkan jenis kelamin paling banyak dijumpai pada jenis kelamin laki-laki yaitu 55,94% dan rentang usia yang paling banyak ditemukan pada usia >59 tahun yaitu 35,15 %. Distribusi Staphylococcus sp. berdasarkan ruang rawat yang paling tinggi dijumpai di ruang ICU yaitu 19,13%. Prevalensi Methicillin-Resistant Staphylocccus aureus (MRSA) dalam penelitian ini yaitu 79,01%.

Kata Kunci: Kultur, spesimen klinik, spesies, Staphylococcus sp.,sensitivitas antibiotik

Staphylococcus is a Gram-positive bacteria that causes infections in humans. This study aims to determine the distribution of Staphylococcus sp. based on clinical specimens, gender, age, and treatment room and to see the level of sensitivity to various types of antibiotics from January 2020 to March 2025 in the Clinical Microbiology Laboratory of Dr. Zainoel Abidin Regional Hospital. This study used a laboratory observation method by collecting data on Staphylococcus sp. culture results from various clinical specimens. Data were collected retrospectively and prospectively. Bacteria were isolated on Blood Agar media and identification and antibiotic sensitivity testing were carried out using the VITEK ® 2 Compact tool. The results of the study obtained 29 species of Staphylococcus from a total of 5,166 identified isolates. The most dominant species distribution was Staphylococcus aureus at 28.69%, followed by Staphylococcus haemolyticus and Staphylococcus hominis ssp hominis at 23.87% and 19.82%, respectively. The distribution of Staphylococcus sp. Based on clinical specimens, the most common type of Staphylococcus sp. was found in blood specimens, namely 52.86%, followed by pus specimens and fluid specimens, respectively 17.13% and 12.27%. The distribution of Staphylococcus sp. based on gender was most commonly found in men, namely 55.94% and the age range most commonly found was in the age of >59 years. namely 35.15%. The distribution of Staphylococcus sp. based on the highest treatment room was found in the ICU, namely 19.13%. The prevalence of Methicillin-Resistant Staphylocccus aureus (MRSA) in this study was 79.01%. Keywords: Culture, clinical specimen, species, Staphylococcus sp., antibiotic sensitivity