Electronic Theses and Dissertation

ABSTRAK
Sapi aceh merupakan plasma nutfah lokal Indonesia yang memiliki nilai strategis, namun
rentan terhadap dampak pemanasan global, khususnya stres akibat panas. Kondisi ini dapat
meningkatkan suhu tubuh dan memicu respons fisiologis berupa sekresi hormon kortisol, yang
berpotensi menurunkan produktivitas dan kesejahteraan hewan. Penelitian ini bertujuan untuk
menganalisis hubungan antara suhu tubuh dengan kadar hormon kortisol pada sapi aceh betina.
Sebanyak 15 ekor sapi aceh betina diamati dalam penelitian ini. Suhu tubuh diukur dari rektal pada
siang hari (12.00–14.00 WIB) bersamaan dengan pengukuran suhu lingkungan, sedangkan sampel
feses dikoleksi satu hari setelah pengukuran suhu tubuh. Sampel feses selanjutnya diektraksi dan
dilakukan pengukuran kadar kortisol menggunakan metode enzyme-linked immunosorbent assay
(ELISA). Data yang diperoleh dianalisis menggunakan uji korelasi Pearson. Hasil menunjukkan
suhu lingkungan (34,60 ± 0,43°C) berkorelasi positif secara signifikan dengan suhu rektal
(37,74 ± 0,75°C) (r = 0,591; p = 0,020), dan suhu rektal juga menunjukkan korelasi positif yang
signifikan dengan kadar kortisol dalam feses (35,59 ± 9,14 ng/g) (r = 0,562; p = 0,029). Dapat
disimpulkan bahwa terdapat hubungan positif yang signifikan antara suhu tubuh dengan kadar
hormon kortisol pada sapi aceh betina.
Kata kunci: kortisol feses, sapi aceh, stres panas, suhu tubuh

ABSTRACT Aceh cattle are a local Indonesian genetic resource with strategic value but are vulnerable to the impacts of global warming, particularly heat stress. This condition can elevate body temperature and trigger physiological responses, including the secretion of the hormone cortisol, which may negatively affect animal productivity and welfare. This study aimed to analyze the relationship between body temperature and cortisol levels in female Aceh cattle. A total of 15 female Aceh cattle were observed in this study. Body temperature was measured rectally during midday (12:00–14:00 WIB) along with environmental temperature, while fecal samples were collected one day after the body temperature measurement. Fecal samples were then extracted and analyzed for cortisol concentration using the ELISA method. The data obtained were analyzed using Pearson correlation. The results showed that environmental temperature (34.60 ± 0.43°C) was positively and significantly correlated with rectal temperature (37.74 ± 0.75°C) (r = 0.591; p = 0.020), and rectal temperature was also positively and significantly correlated with fecal cortisol levels (35.59 ± 9.14 ng/g) (r = 0.562; p = 0.029). It can be concluded that there is a significant positive relationship between body temperature and cortisol levels in female Aceh cattle. Keywords: Aceh cattle, body temperature, fecal cortisol, heat stress