Electronic Theses and Dissertation

Spermatogenesis merupakan proses kompleks yang sangat bergantung pada regulasi hormonal, khususnya oleh follicle stimulating hormone (FSH) dan luteinizing hormone (LH). Penelitian ini bertujuan mengevaluasi pengaruh ekstrak hipofisa sapi (EHS), yang mengandung FSH dan LH, terhadap perkembangan spermatid pada testis tikus putih jantan (Rattus norvegicus). Sebanyak 21 ekor tikus Wistar jantan dibagi dalam tujuh kelompok perlakuan, yaitu P0 (kontrol, NaCl 0,9%) dan P1–P6 yang menerima injeksi EHS selama 1–6 minggu. Dosis EHS yang diberikan adalah 3 IU/ekor, tiga kali per minggu. Setelah periode perlakuan, testis diambil dan dianalisis secara histologis menggunakan pewarnaan Periodic Acid–Schiff (PAS) untuk mengidentifikasi tahap perkembangan spermatid (round, elongating, dan elongated). Data dianalisis menggunakan analysis of varian (ANOVA) dan dilanjutkan dengan uji Tukey HSD. Hasil penelitian menunjukkan bahwa pemberian EHS meningkatkan jumlah round spermatid secara signifikan pada minggu pertama (P1), namun mengalami penurunan setelah minggu keempat. Jumlah elongating spermatid tidak menunjukkan perbedaan signifikan antar kelompok. Jumlah elongated spermatid tertinggi ditemukan pada minggu kedua (P2). Disimpulkan bahwa EHS berpotensi meningkatkan perkembangan spermatid, terutama pada fase awal, namun peningkatan durasi pemberian dapat menurunkan efektivitas biologisnya.

Kata kunci: spermatogenesis, ekstrak hipofisa sapi, FSH, LH, spermatid, tikus putih

Spermatogenesis is a highly complex process that is critically regulated by hormonal signals, particularly follicle-stimulating hormone (FSH) and luteinizing hormone (LH). This study aimed to evaluate the effect of bovine pituitary extract (BPE), which contains both FSH and LH, on spermatid development in the testes of male Wistar rats (Rattus norvegicus). A total of 21 male rats were randomly assigned to seven treatment groups: P0 (control, receiving 0.9% NaCl) and P1–P6, which received intraperitoneal injections of BPE for durations ranging from one to six weeks. The BPE was administered at a dose of 3 IU/rat, three times per week. Following the treatment period, testes were collected and subjected to histological analysis using Periodic Acid–Schiff (PAS) staining to identify the stages of spermatid development (round, elongating, and elongated). Data were analyzed using analysis of variance (ANOVA), followed by Tukey’s HSD post hoc test. The findings indicated that BPE administration significantly increased the number of round spermatids in the first week (P1); however, a decline was observed after the fourth week. The number of elongating spermatids did not differ significantly among the groups. The highest number of elongated spermatids was observed in the second week (P2). In conclusion, BPE demonstrates potential in enhancing spermatid development, particularly during the early stages of treatment, though prolonged administration may diminish its biological efficacy. . Keyword: spermatogenesis, Bovine pituitary extract, FSH, LH, spermatid, white rat