Electronic Theses and Dissertation

Kualitas spermatozoa dipengaruhi oleh berbagai faktor, termasuk mineral esensial magnesium (Mg) dan kalsium (Ca). Namun informasi mengenai hubungan kadar Mg dan Ca dengan kualitas spermatozoa pada sapi aceh masih terbatas. Penelitian ini bertujuan untuk menganalisis hubungan antara kadar Mg dan Ca dalam seminal plasma dengan kualitas spermatozoa pada sapi aceh jantan. Sperma dikoleksi menggunakan teknik vagina buatan dari 6 ekor sapi dan dievaluasi secara makroskopis (volume, warna, konsistensi, bau, pH) serta mikroskopis (gerakan massa, motilitas, viabilitas, abnormalitas, konsentrasi). Kadar Mg dan Ca dalam seminal plasma diukur menggunakan metode Anatomic Absorbtion Spectrophotometer (AAS). Data dianalisis secara deskriptif dan diuji menggunakan korelasi person. Hasil penelitian menunjukkan bahwa kadar Mg dalam seminal plasma berkorelasi positif dan signifikan dengan motilitas spermatozoa (r = 0,849, p = 0,032), tetapi tidak berhubungan signifikan dengan parameter kualitas spermatozoa lainnya. Sementara itu, kadar Ca dalam seminal plasma tidak menunjukkan hubungan signifikan dengan kualitas spermatozoa, meskipun terdapat kecenderungan peningkatan kadar Ca yang terkait dengan peningkatan abnormalitas spermatozoa ( r = 0,794, p = 0,059). Dapat disimpulkan bahwa terdapat korelasi positif dan signifikan antara Mg dalam seminal plasma dengan motilitas spermatozoa sapi aceh, sedangkan Ca tidak menunjukkan korelasi signifikan dengan kualitas spermatozoa.

Kata kunci : Sapi Aceh, Kualitas Spermatozoa, Makro Mineral, Magnesium, Kalsium.

Semen quality is influenced by various factors, including essential minerals such as magnesium (Mg) and calcium (Ca). However, information regarding the relationship between Mg and Ca levels and spermatozoa quality in Aceh cattle remains limited. This study aims to analyze the relationship between Mg and Ca levels in seminal plasma and spermatozoa quality in Aceh bulls. Semen was collected using the artificial vagina technique from 6 cows and evaluated macroscopically (volume, color, consistency, odor, pH) and microscopically (mass movement, motility, viability, abnormalities, concentration). Mg and Ca levels in seminal plasma were measured using Atomic Absorption Spectrophotometry (AAS). Data were analyzed descriptively and tested using Pearson correlation. The results showed that Mg levels in seminal plasma positively and significantly correlated with spermatozoa motility (r = 0.849; p = 0.032) but were not significantly correlated with other semen quality parameters. Meanwhile, Ca levels in seminal plasma were not significantly correlated with any semen quality parameters, although there was a tendency for increased Ca levels to be associated with increased spermatozoa abnormalities (r = 0.794; p = 0.059). It can be concluded that Mg in seminal plasma has a positive and significant relationship with spermatozoa motility in aceh bulls, whereas Ca does not show a significant relationship with spermatozoa quality. Keywords: Aceh Cattle, Spermatozoa, Macro Minerals, Magnesium, Calcium.