Electronic Theses and Dissertation

Aktinobakteri merupakan kelompok mikroorganisme yang diisolasi dari tanah dan dikenal mampu menghasilkan berbagai senyawa bioaktif, salah satunya sebagai inhibitor lipase. Penelitian ini bertujuan untuk mengetahui aktivitas aktinobakteri tanah dalam menghambat lipase, menganalisis senyawa bioaktif yang dihasilkan dan mengidentifikasi karakteristik isolat terpilih secara molekuler. Uji aktivitas inhibitor lipase dilakukan secara in vitro dengan menambahkan supernatan isolat aktinobakteri tanah, substrat (Phosphate Buffered Seline 0,1 M (pH 8), tween 80%, minyak zaitun) dan enzim lipase. Nilai absorbansinya diukur menggunakan Spektrofotometer UV-vis. Analisis senyawa bioaktif dari isolat terpilih dilakukan menggunakan Gas Chromatography–Mass Spectrometry (GC-MS). Identifikasi karakteristik isolat aktinobakteri tanah dilakukan berdasarkan gen 16S rRNA. Hasil yang diperoleh menunjukkan isolat ATS14 mempu menghambat lipase sebesar 98,26%. Analisis GC-MS mendeteksi sebanyak 13 senyawa volatil dengan asam oleat (Oleid acid) yang berperan sebagai inhibitor lipase. Isolat ATS14 memiliki kemiripan sekuens sebesar 99,92% dengan Streptomyces sp. strain S15_SBA. Kemampuan senyawa bioaktif dari isolat ATS14 dalam menghambat lipase dapat menjadi sumber alami senyawa inhibitor lipase dan berpotensi untuk dikembangkan sebagai agen antiobesitas.

Kata kunci: Aktinobakteri, Inhibitor lipase, Gas Chromatography–Mass Spectrometry (GC-MS), Gen 16S rRNA, Streptomyces sp.

Actinobacteria are a group of microorganisms isolated from soil and are known to produce various bioactive compounds, one of which is a lipase inhibitor. This study aims to determine the activity of soil actinobacterial in inhibiting lipase, analyze the bioactive compounds produced and identify the characteristics of selected isolates molecularly. Lipase inhibitor activity test was conducted in vitro by adding supernatant of soil actinobacterial isolates, substrate (0.1 M Phosphate Buffered Seline (pH 8), 80% tween, olive oil) and lipase enzyme. The absorbance values were measured using UV-vis spectrophotometer. Analysis of bioactive compounds from selected isolates was performed using Gas Chromatography-Mass Spectrometry (GC-MS). Identification of the characteristics of soil actinobacterial isolates was carried out based on the 16S rRNA gene. The results obtained showed that isolate ATS14 had the ability to inhibit lipase by 98.26%. GC-MS analysis detected as many as 13 volatile compounds with oleic acid (Oleid acid) which acts as a lipase inhibitor. Isolate ATS14 has a sequence similarity of 99.92% with Streptomyces sp. strain S15_SBA. The ability of bioactive compounds from isolate ATS14 in inhibiting lipase can be a natural source of lipase inhibitor compounds and has the potential to be developed as an antiobesity agent. Keywords:Actinobacteria, Lipase inhibitor, Gas Chromatography–Mass Spectrometry (GC-MS), 16S rRNA gene, Streptomyces sp.