Electronic Theses and Dissertation

Serai dapur (Cymbopogon citratus) merupakan tanaman yang banyak digunakan secara tradisional sebagai pengobatan di Indonesia. Minyak atsiri adalah salah satu komponen utama dari C. citratus yang telah terbukti memiliki aktivitas farmakologis, salah satunya sebagai antiinflamasi. Tujuan dari penelitian ini adalah menguji aktivitas antiinflamasi minyak atsiri dari bagian aerial C. citratus. Minyak atsiri diperoleh dengan metode hidrodistilasi dan karakterisasi dilakukan melalui uji organoleptis serta analisis senyawa kimia menggunakan instrumen GC-MS. Uji aktivitas antiinflamasi
dilakukan menggunakan metode ELISA melalui inhibisi prostaglandin E2 (PGE2) pada sel RAW264.7 yang diinduksi lipopolisakarida (LPS). Hasil penelitian ini menunjukkan bahwa minyak atsiri C. citratus memiliki persen rendemen sebesar
0,492%, berbentuk cairan jernih, berwarna kuning pucat, beraroma segar khas. Hasil GC-MS menunjukkan bahwa minyak atsiri C. citratus terdiri dari 51 jenis senyawa dan didominasi oleh trans-citral (34,77%), cis-citral (20,61%), alpha-chamipinene
(6,82%) dan neric acid (4,49%). Uji aktivitas antiinflamasi menunjukkan bahwa pada konsentrasi 3.125, 6.25, 12.5, 25 dan 50 ug/mL minyak atsiri C. citratus tidak memberikan efek inhibisi PGE2

Lemongrass (Cymbopogon citratus) is a plant that is widely used traditionally as a medicine in Indonesia. Essential oil is one of the main components of C. citratus that has been shown to have pharmacological activities, one of which is anti-inflammatory. The purpose of this study was to test the anti-inflammatory activity of essential oil from the aerial part of C. citratus. Essential oil was obtained by hydrodistillation method and characterization was done through organoleptic test and chemical compound analysis using GC-MS instrument. Anti-inflammatory activity test was conducted using ELISA method through prostaglandin E2 (PGE2) inhibition in lipopolysaccharide (LPS)-induced RAW264.7 cells. The results of this study showed that C. citratus essential oil has a percent yield of 0.492%, in the form of clear liquid, pale yellow in color, with a distinctive fresh aroma. GC-MS results show that C. citratus essential oil consists of 51 types of compounds and is dominated by trans citral (34.77%), cis-citral (20.61%), alpha-chamipinene (6.82%) and neric acid (4.49%). Anti-inflammatory activity test showed that at concentrations of 3.125, 6.25, 12.5, 25 and 50 ug/mL, C. citratus essential oil did not inhibit PGE2 in RAW264.7 cells. This occurred due to several factors in the testing process.